Linear poly(amidoamine)s (PAAs) have been designed to exhibit minimal non-specific toxicity, display pH-dependent membrane lysis and deliver genes and toxins in vitro. The aim of this study was to measure PAA cellular uptake using ISA1-OG (and as a ref. ISA23-OG) in B16F10 cells in vitro and, by subcellular fractionation, quantitate intracellular trafficking of 125I-labeled ISA1-tyr in liver cells after i.v. administration to rats. The effect of time after administration (0.5-3 h) and ISA1 dose (0.04-100 mg/kg) on trafficking, and vesicle permeabilization (N-acetyl-b-D-glucosaminidase (NAG)
Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo / S.C.W. Richardson, N. G. Pattrick, N. Lavignac, P. Ferruti, R. Duncan. - In: JOURNAL OF CONTROLLED RELEASE. - ISSN 0168-3659. - 142:1(2010), pp. 78-88. [10.1016/j.jconrel.2009.09.025]
Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo
P. FerrutiPenultimo
;
2010
Abstract
Linear poly(amidoamine)s (PAAs) have been designed to exhibit minimal non-specific toxicity, display pH-dependent membrane lysis and deliver genes and toxins in vitro. The aim of this study was to measure PAA cellular uptake using ISA1-OG (and as a ref. ISA23-OG) in B16F10 cells in vitro and, by subcellular fractionation, quantitate intracellular trafficking of 125I-labeled ISA1-tyr in liver cells after i.v. administration to rats. The effect of time after administration (0.5-3 h) and ISA1 dose (0.04-100 mg/kg) on trafficking, and vesicle permeabilization (N-acetyl-b-D-glucosaminidase (NAG)
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