Cytospin preparations of fine-needle aspirates from 21 dogs with peripheral lymphadenopathy (18 with lymphoma and three with lymph node hyperplasia) were studied by combining morphologic and immunocytochemical analysis. Fine-needle aspirates were taken from at least two enlarged lymph nodes, and the diagnosis was based on air-dried smears stained with May-Grünwald Giemsa. Fine-needle aspiration biopsy always provided an adequate quality and quantity of cells to perform morphologic and immunologic studies. Immunophenotyping was performed on cytospin preparations with a panel of eight monoclonal antibodies specific for canine cell surface antigens and one rabbit polyclonal antibody (A452) against human CD3, which cross-reacts with dog antigen. The immunocytochemical study resulted in the diagnosis of 14 B-cell lymphomas (CD21+, CD3-) and three T-cell lymphomas (all CD3+, two CD8+). One lymphoma lacked surface antigens specific for the B- or T-cell lineage and was classified as non-B-non-T lymphoma (CD21-, CD3-, CD4-, CD8-). The monoclonal antibodies CA12.10C12, CA4.1D3, and CA1D6 and the polyclonal antibody A452, used as a group, appeared to be the most useful reagents to suggest lymphoid origin and to discriminate between T- and B-cell phenotype. Cytospin preparations in combination with immunocytochemistry provided a practical, economical, and accurate method for the diagnosis and phenotyping of canine lymphoma.
Canine lymphoma : immunocytochemical analysis of fine needle-aspiration biopsy / M. Caniatti, P. Roccabianca, E. Scanziani, S. Paltrinieri, P.F. Moore. - In: VETERINARY PATHOLOGY. - ISSN 0300-9858. - 33:2(1996 Mar), pp. 204-212.
|Titolo:||Canine lymphoma : immunocytochemical analysis of fine needle-aspiration biopsy|
CANIATTI, MARIO (Primo)
ROCCABIANCA, PAOLA (Secondo)
PALTRINIERI, SAVERIO (Penultimo)
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
|Data di pubblicazione:||mar-1996|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1177/030098589603300210|
|Appare nelle tipologie:||01 - Articolo su periodico|