Intense physical exercise activates the hypothalamic-pituitary-adrenocortical axis but little is known about changes in glucocorticoid sensitivity at the target cell level. No data are available on the acute effects of exercise on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 1 activity, which generates biologically active cortisol from inactive cortisone and is expressed also in skeletal muscle. Fifteen healthy, trained males (age mean +/- SE 28 +/- 1) were assessed on three non-consecutive days: at rest, during an endurance and strength sessions. During each session, between 1000 and 1600 hours, 6-h urine and four salivary samples were collected. Urinary total tetrahydrocortisol (THF) + alloTHF, tetrahydrocortisone (THE), cortisol (F) and cortisone (E) were measured with HPLC-tandem mass spectrometry; urinary-unconjugated F and E were measured by HPLC-UV. Salivary cortisol and interleukin (IL)-6 were measured by RIA and ELISA, respectively. Both endurance and strength exercises caused an increase in (THF + alloTHF)/THE ratio (mean +/- SE 1.90 +/- 0.07 and 1.82 +/- 0.05 vs. 1.63 +/- 0.06, P < 0.01 and P = 0.03, respectively), consistent with increased systemic 11beta-HSD type 1 activity. No relationship was found with age, BMI, VO(2max) maximal power load or perceived exertion. No significant change was apparent in F/E ratio, an index of 11beta-HSD type 2 activity. No effect of exercise on salivary cortisol and IL-6 was observed, whereas a significant effect of sampling time was found. Intense physical exercise acutely increases systemic 11beta-HSD type 1 activity in humans. Such an increase may lead to higher cortisol concentration in target tissues, notably in skeletal muscle where it could contribute to limit exercise-induced muscle inflammatory response.
Intense physical exercise increases systemic 11β-hydroxysteroid dehydrogenase type 1 activity in healthy adult subjects / A. Dovio, E. Roveda, C. Sciolla, A. Montaruli, A. Raffaelli, A. Saba, G. Calogiuri, S. De Francia, P. Borrione, P. Salvatori, F. Carandente, A. Angeli. - In: EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY. - ISSN 1439-6319. - 108:4(2010 Mar), pp. 681-687.
|Titolo:||Intense physical exercise increases systemic 11β-hydroxysteroid dehydrogenase type 1 activity in healthy adult subjects|
ROVEDA, ELIANA (Secondo)
CARANDENTE, FRANCA (Penultimo)
|Parole Chiave:||11β-Hydroxysteroid dehydrogenase activity; Cortisol; Endurance; Interleukin 6; Strength|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
Settore BIO/16 - Anatomia Umana
|Data di pubblicazione:||mar-2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00421-009-1265-5|
|Appare nelle tipologie:||01 - Articolo su periodico|