The measurement of plasma warfarin is required to investigate non-compliance, resistance to anticoagulation, drug metabolism and pharmacokinetic. Methods so far described are based on extraction of warfarin from plasma followed by reversed-phase HPLC. Extraction is the crucial step and may be performed in liquid- or solid-phase. The latter requires the preparation of columns, which makes the procedure variable. We investigated the suitability of the ready-for-use commercial cartridges for sample preparation. The method displayed between-run CV of 11.8%. Recovery was 99%. The coefficients of correlation between warfarin concentration in 50 patients and weekly dosage or INR were 0.55 (p<0.0001) or 0.25 (p=0.079).
Measurement of warfarin in plasma by high performance liquid chromatography (HPLC) and its correlation with the international normalized ratio / R. Lombardi, V. Chantarangkul, M. Cattaneo, A. Tripodi. - In: THROMBOSIS RESEARCH. - ISSN 0049-3848. - 111:4-5(2003), pp. 281-284.
Measurement of warfarin in plasma by high performance liquid chromatography (HPLC) and its correlation with the international normalized ratio
M. CattaneoPenultimo
;A. TripodiUltimo
2003
Abstract
The measurement of plasma warfarin is required to investigate non-compliance, resistance to anticoagulation, drug metabolism and pharmacokinetic. Methods so far described are based on extraction of warfarin from plasma followed by reversed-phase HPLC. Extraction is the crucial step and may be performed in liquid- or solid-phase. The latter requires the preparation of columns, which makes the procedure variable. We investigated the suitability of the ready-for-use commercial cartridges for sample preparation. The method displayed between-run CV of 11.8%. Recovery was 99%. The coefficients of correlation between warfarin concentration in 50 patients and weekly dosage or INR were 0.55 (p<0.0001) or 0.25 (p=0.079).Pubblicazioni consigliate
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