A microbial consortium selected on 2-chlorobenzoate was shown to be able to also utilise 4-chlorobenzoate and 2,5-dichlorobenzoate as sole carbon source. The consortium adapted to grow on 4-chlorobenzoate, degraded the carbon source via protocatechuate, whereas the same consortium degraded 2-chlorobenzoate and 2,5-dichlorobenzoate via 1,2- or 1,6-dioxygenation of the chlorinated ring. Moreover, no significant effects on the microbial growth due to the presence of chlorobenzoate mixtures were observed when 4-chlorobenzoate was the carbon source. Instead, when meta-substituted chlorobenzoates were added as co-substrates to 2,5-dichlorobenzoate, the growth of the consortium was totally inhibited, just as when the culture utilised for growth 2-chlorobenzoate. Uptake experiments with 2-chlorobenzoate-grown resting cells showed that 3-chlorobenzoate, 2,3-dichlorobenzoate and 2,3,5-trichlorobenzoate competed with 2-chlorobenzoate, entering the resting cells both preferentially and faster than the growth substrate, that was also impeded to enter. Also 3,4-dichlorobenzoate and 3,5-dichlorobenzoate hindered the uptake of the growth substrate (2-chlorobenzoate and 2,5-dichlorobenzoate, respectively), but they did not enter themselves the cells. Finally, 3,5-dichlorobenzoate neither entered the 4-chlorobenzoate-grown cells nor hindered 4-chlorobenzoate uptake. The relationships between growth inhibiting effects and chlorosubstituent position on the aromatic ring of the chlorobenzoates supplied as co-substrates are discussed.

Inhibitory Mechanisms by Chlorobenzoate Mixtures in Chlorobenzoate-degrading Microorganisms / G. Baggi, S. Bernasconi, M. Zangrossi. - In: ANNALS OF MICROBIOLOGY. - ISSN 1590-4261. - 59:4(2009), pp. 657-662. [10.1007/BF03179205]

Inhibitory Mechanisms by Chlorobenzoate Mixtures in Chlorobenzoate-degrading Microorganisms

S. Bernasconi
Secondo
;
M. Zangrossi
Ultimo
2009

Abstract

A microbial consortium selected on 2-chlorobenzoate was shown to be able to also utilise 4-chlorobenzoate and 2,5-dichlorobenzoate as sole carbon source. The consortium adapted to grow on 4-chlorobenzoate, degraded the carbon source via protocatechuate, whereas the same consortium degraded 2-chlorobenzoate and 2,5-dichlorobenzoate via 1,2- or 1,6-dioxygenation of the chlorinated ring. Moreover, no significant effects on the microbial growth due to the presence of chlorobenzoate mixtures were observed when 4-chlorobenzoate was the carbon source. Instead, when meta-substituted chlorobenzoates were added as co-substrates to 2,5-dichlorobenzoate, the growth of the consortium was totally inhibited, just as when the culture utilised for growth 2-chlorobenzoate. Uptake experiments with 2-chlorobenzoate-grown resting cells showed that 3-chlorobenzoate, 2,3-dichlorobenzoate and 2,3,5-trichlorobenzoate competed with 2-chlorobenzoate, entering the resting cells both preferentially and faster than the growth substrate, that was also impeded to enter. Also 3,4-dichlorobenzoate and 3,5-dichlorobenzoate hindered the uptake of the growth substrate (2-chlorobenzoate and 2,5-dichlorobenzoate, respectively), but they did not enter themselves the cells. Finally, 3,5-dichlorobenzoate neither entered the 4-chlorobenzoate-grown cells nor hindered 4-chlorobenzoate uptake. The relationships between growth inhibiting effects and chlorosubstituent position on the aromatic ring of the chlorobenzoates supplied as co-substrates are discussed.
Chlorobenzoate mixtures; Competition; Growth inhibition; Microbial consortium; Uptake
Settore CHIM/06 - Chimica Organica
Settore AGR/16 - Microbiologia Agraria
2009
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/144987
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