This report describes the unusual case of a patient affected by Crohn’s disease suffering from intestinal obstruction with recurrent occlusive symptoms not due to the intestinal disease, but to the presence of two calcified foreign bodies in the pelvis. The stones were surgically removed and analysed by reverse-phase liquid chromatography coupled to UV diode array detection and mass spectrometry (LC-UV-DAD-MS/MS), Chromatoprobe-MS/MS and by Fourier-transform-infrared spectroscopy (FT-IR) techniques. The combined mass spectrometric approaches allowed unequivocally to identify 5-aminosalicylic acid (5-ASA) in stone 1, and to demonstrate that its formation was due to an unmodified 5-ASA tablet, a formulation that must undergo complete dissolution in the small bowel. The second stone was constituted by a solid layer (no solvent-extractable material) identified by FT-IR as a polystyrene fragment. This indicates that accidental ingestion of a plastic material, followed by its calcification, was responsible for its formation.
LC-MS/MS and FT-IR analyses of stones from a patient with Crohn’s disease: a case report / M. Orioli, C. Marinello, R. Cozzi, L.P. Piodi, M. Carini. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 35:5(2004), pp. 1263-1272.
|Titolo:||LC-MS/MS and FT-IR analyses of stones from a patient with Crohn’s disease: a case report|
ORIOLI, MARICA (Primo)
MARINELLO, CRISTINA (Secondo)
CARINI, MARINA (Ultimo)
|Parole Chiave:||5-Aminosalicylic acid; Crohn's disease; Fourier-transform-infrared spectroscopy; Ileal obstruction; Liquid chromatography tandem mass spectrometry; Polystyrene; Stones|
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
|Data di pubblicazione:||2004|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jpba.2004.03.016|
|Appare nelle tipologie:||01 - Articolo su periodico|