b-Amyloid precursor protein (APP) mutations cause familial Alzheimer’s disease with nearly complete penetrance. We found an APP mutation [alanine-673→valine-673 (A673V)] that causes disease only in the homozygous state, whereas heterozygous carriers were unaffected, consistent with a recessive Mendelian trait of inheritance. The A673V mutation affected APP processing, resulting in enhanced b-amyloid (Ab) production and formation of amyloid fibrils in vitro. Coincubation of mutated and wild-type peptides conferred instability on Ab aggregates and inhibited amyloidogenesis and neurotoxicity. The highly amyloidogenic effect of the A673V mutation in the homozygous state and its anti-amyloidogenic effect in the heterozygous state account for the autosomal recessive pattern of inheritance and have implications for genetic screening and the potential treatment of Alzheimer’s disease.
|Titolo:||A Recessive Mutation in the APP Gene with Dominant-Negative Effect on Amyloidogenesis|
|Settore Scientifico Disciplinare:||Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)|
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||10.1126/science.1168979|
|Appare nelle tipologie:||01 - Articolo su periodico|