The worldwide emergence of antimicrobial-resistant bacteria poses a serious threat to human health. The understanding of the mechanisms of antimicrobial resistance is extremely important for the control of these bacteria. Multidrug-resistant bacteria have frequently been reported, but information regarding proteome and its roles in the regulation of multidrug resistance is not yet available. In the current study, proteomic methodologies were used to characterize the proteome of multidrug-resistant Escherichia coli. Strains of Escherichia coli O157, O128, O111 and O26 isolated from buffalo feces and tested using antibiotic disk susceptibility methods were analyzed. Altered proteins of these E. coli strains were identified by 2-D gel based proteomic methodologies. The changes at the protein expression level detected by 2-D gel electrophoresis were validated by Mass Spectrometry. The information obtained from this study provides novel insights into mechanisms of antibiotic resistance.
Proteomic analysis of multidrug resistance in Escherichia coli / S. Lauzi, F. Deriu, A. Soggiu, L. Bonizzi, P. Roncada. ((Intervento presentato al 5. convegno Med-Vet-Net Annual Scientific Meeting tenutosi a Madrid nel 2009.
Proteomic analysis of multidrug resistance in Escherichia coli
S. LauziPrimo
;F. DeriuSecondo
;A. Soggiu;L. BonizziPenultimo
;
2009
Abstract
The worldwide emergence of antimicrobial-resistant bacteria poses a serious threat to human health. The understanding of the mechanisms of antimicrobial resistance is extremely important for the control of these bacteria. Multidrug-resistant bacteria have frequently been reported, but information regarding proteome and its roles in the regulation of multidrug resistance is not yet available. In the current study, proteomic methodologies were used to characterize the proteome of multidrug-resistant Escherichia coli. Strains of Escherichia coli O157, O128, O111 and O26 isolated from buffalo feces and tested using antibiotic disk susceptibility methods were analyzed. Altered proteins of these E. coli strains were identified by 2-D gel based proteomic methodologies. The changes at the protein expression level detected by 2-D gel electrophoresis were validated by Mass Spectrometry. The information obtained from this study provides novel insights into mechanisms of antibiotic resistance.Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.