The purpose of this study was to determine the fate and the effects of undifferentiated embryonic stem cells (ESCs) in mice after contusive lesion of the spinal cord (SCI). Reproducible traumatic lesion to the cord was performed at T8 level by means of the Infinite Horizon Device, and was followed by intravenous injection of one million of undifferentiated ESCs through the tail vein within 2 h from the lesion. The ESCs-treated animals showed a significant improvement of the recovery of motor function 28 days after lesion, with an average score of 4.61±0.13 points of the Basso Mouse Scale (n=14), when compared to the average score of vehicle treated mice, 3.58±0.23 (n=10). The number of identified ESCs found at the lesion site was 0.6% of the injected cells at 1 week after transplantation, and further reduced to 0.04% at 1 month. It is, thus, apparent that the promoted hind-limb recovery cannot be correlated to a substitution of the lost tissue performed by the exogenous ESC. The extensive evaluation of production of several neuroprotective and inflammatory cytokines did not reveal any effect by ESC-treatment, but unexpectedly the number of invading macrophages and neutrophils was greatly reduced. This may explain the improved preservation of lesion site ventral myelin, at both 1 week (29±11%) and 1 month (106±14%) after injury. No teratoma formation was observed, although an inappropriate colonization of the sacral cord by differentiated nestinand β-tubulin III-positive ESCs was detected

Embryonic stem cells promote motor recovery and affect inflammatory cell infiltration in spinal cord injured mice / D. Bottai, D. Cigognini, L. Madaschi, R. Adami, E. Nicora, M. Menarini, A.M. Di Giulio, A. Gorio. - In: EXPERIMENTAL NEUROLOGY. - ISSN 0014-4886. - 223:2(2010 Jun), pp. 452-463.

Embryonic stem cells promote motor recovery and affect inflammatory cell infiltration in spinal cord injured mice

D. Bottai
Primo
;
D. Cigognini
Secondo
;
L. Madaschi;R. Adami;E. Nicora;A.M. Di Giulio
Penultimo
;
A. Gorio
Ultimo
2010

Abstract

The purpose of this study was to determine the fate and the effects of undifferentiated embryonic stem cells (ESCs) in mice after contusive lesion of the spinal cord (SCI). Reproducible traumatic lesion to the cord was performed at T8 level by means of the Infinite Horizon Device, and was followed by intravenous injection of one million of undifferentiated ESCs through the tail vein within 2 h from the lesion. The ESCs-treated animals showed a significant improvement of the recovery of motor function 28 days after lesion, with an average score of 4.61±0.13 points of the Basso Mouse Scale (n=14), when compared to the average score of vehicle treated mice, 3.58±0.23 (n=10). The number of identified ESCs found at the lesion site was 0.6% of the injected cells at 1 week after transplantation, and further reduced to 0.04% at 1 month. It is, thus, apparent that the promoted hind-limb recovery cannot be correlated to a substitution of the lost tissue performed by the exogenous ESC. The extensive evaluation of production of several neuroprotective and inflammatory cytokines did not reveal any effect by ESC-treatment, but unexpectedly the number of invading macrophages and neutrophils was greatly reduced. This may explain the improved preservation of lesion site ventral myelin, at both 1 week (29±11%) and 1 month (106±14%) after injury. No teratoma formation was observed, although an inappropriate colonization of the sacral cord by differentiated nestinand β-tubulin III-positive ESCs was detected
i.v. cell injection ; confocal microscopy inflammation ; stem cells ; traumatic spinal cord injury
Settore BIO/14 - Farmacologia
Settore BIO/11 - Biologia Molecolare
Settore MED/26 - Neurologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/141403
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