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EnglishExpression of L-selectin was determined by single- and two-colour immunofluorescence on granulocytes, peripheral blood mononuclear cells (PBMC) and blasts of bovine origin by means of a monoclonal antibody IVA94 which recognizes bovine L-selectin (CD62L). Cells were separated from peripheral blood of healthy cattle and colleagues infected with bovine leukaemia virus (BLV). BLV-infected animals comprised lymphocytotic and non-lymphocytotic cows. L-selectin was expressed on 90–98 % of granulocytes in all tested animals. The percentage of PBMC expressing L-selectin was lower in cattle with persistent lymphocytosis than in non-lymphocytotic or BLV-free cattle, and inversely correlated with lymphocyte counts. The ratio of B lymphocytes stained for L-selectin was significantly decreased from 60.2 ± 1.9 % in BLV-free cattle to 43.8 ± 3.6 and 22.5 ± 5.7 % in non-lymphocytotic and lymphocytotic cattle, respectively. B-lymphocytes stained for L-selectin exhibited about 50 % reduction in L-selectin expression in BLV-infected cattle compared with BLV-free cattle, as judged by the mean fluorescence intensity (MFI). The percentage of L-selectin-positive PBMC not bearing surface immunoglobulin M (predominantly T lymphocytes) was comparable in BLV-free and BLV-infected cattle. However, L-selectin expression on T lymphocytes was reduced (about 50 %) in BLV-infected cattle, as judged by the MFI. We suppose that BLV infection results in a decreased L-selectin expression on lymphocytes, and accordingly, it may contribute to deregulation of the host immune system
| Titolo: | Decreased expression of L-Selectin (CD62L) on lymphocytes in enzootic bovine leukemia |
| Autori: | PONTI, WILMA (Ultimo) |
| Settore Scientifico Disciplinare: | Settore VET/05 - Malattie Infettive degli Animali Domestici |
| Data di pubblicazione: | mar-2000 |
| Rivista: | |
| Tipologia: | Article (author) |
| Digital Object Identifier (DOI): | 10.1046/j.1439-0450.2000.00326.x |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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