The NMDA receptor complex represents a key molecular element in the pathogenesis of long-term synaptic changes and motor abnormalities in Parkinson's disease (PD). Here we show that NMDA receptor 1 (NR1) subunit and postsynaptic density (PSD)-95 protein levels are selectively reduced in the PSD of dopamine (DA)-denervated striata. These effects are accompanied by an increase in striatal levels of {alpha}Ca2+-calmodulin-dependent protein kinase II ({alpha}CaMKII) autophosphorylation, along with a higher recruitment of activated {alpha}CaMKII to the regulatory NMDA receptor NR2A-NR2B subunits. Acute treatment of striatal slices with R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride, but not with L-sulpiride, mimicked the effect of DA denervation on both {alpha}CaMKII autophosphorylation and corticostriatal synaptic plasticity. In addition to normalizing {alpha}CaMKII autophosphorylation levels as well as assembly and anchoring of the kinase to the NMDA receptor complex, intrastriatal administration of the CaMKII inhibitors KN-93 (N-[2-[[[3-(4-chlorophenyl)-2-propenyl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4-methoxybenzenesulfonamide) and antennapedia autocamtide-related inhibitory peptide II is able to reverse both the alterations in corticostriatal synaptic plasticity and the deficits in spontaneous motor behavior that are found in an animal model of PD. The same beneficial effects are produced by a regimen of L-3,4-dihydroxyphenylalanine (L-DOPA) treatment, which is able to normalize {alpha}CaMKII autophosphorylation. These data indicate that abnormal {alpha}CaMKII autophosphorylation plays a causal role in the alterations of striatal plasticity and motor behavior that follow DA denervation. Normalization of CaMKII activity may be an important underlying mechanism of the therapeutic action of L-DOPA in PD.

Abnormal Ca2+-calmodulin-dependent protein kinase II function mediates synaptic and motor deficits in experimental parkinsonism / B. Picconi, F. Gardoni , D. Centonze, D. Mauceri, M.A. Cenci, G. Bernardi, P. Calabresi, M. Di Luca. - In: THE JOURNAL OF NEUROSCIENCE. - ISSN 0270-6474. - 24:23(2004), pp. 5283-5291.

Abnormal Ca2+-calmodulin-dependent protein kinase II function mediates synaptic and motor deficits in experimental parkinsonism

F. Gardoni
Secondo
;
D. Mauceri;M. Di Luca
Ultimo
2004

Abstract

The NMDA receptor complex represents a key molecular element in the pathogenesis of long-term synaptic changes and motor abnormalities in Parkinson's disease (PD). Here we show that NMDA receptor 1 (NR1) subunit and postsynaptic density (PSD)-95 protein levels are selectively reduced in the PSD of dopamine (DA)-denervated striata. These effects are accompanied by an increase in striatal levels of {alpha}Ca2+-calmodulin-dependent protein kinase II ({alpha}CaMKII) autophosphorylation, along with a higher recruitment of activated {alpha}CaMKII to the regulatory NMDA receptor NR2A-NR2B subunits. Acute treatment of striatal slices with R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride, but not with L-sulpiride, mimicked the effect of DA denervation on both {alpha}CaMKII autophosphorylation and corticostriatal synaptic plasticity. In addition to normalizing {alpha}CaMKII autophosphorylation levels as well as assembly and anchoring of the kinase to the NMDA receptor complex, intrastriatal administration of the CaMKII inhibitors KN-93 (N-[2-[[[3-(4-chlorophenyl)-2-propenyl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4-methoxybenzenesulfonamide) and antennapedia autocamtide-related inhibitory peptide II is able to reverse both the alterations in corticostriatal synaptic plasticity and the deficits in spontaneous motor behavior that are found in an animal model of PD. The same beneficial effects are produced by a regimen of L-3,4-dihydroxyphenylalanine (L-DOPA) treatment, which is able to normalize {alpha}CaMKII autophosphorylation. These data indicate that abnormal {alpha}CaMKII autophosphorylation plays a causal role in the alterations of striatal plasticity and motor behavior that follow DA denervation. Normalization of CaMKII activity may be an important underlying mechanism of the therapeutic action of L-DOPA in PD.
6-OHDA; Parkinson's disease; CaMKII; LTP; rat; striatum
Settore BIO/14 - Farmacologia
2004
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/13305
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