Flavescence dorée (FD) of grapevine is a serious disease caused by phytoplasmas. Currently available protocols for phytoplasma detection in grapevine are complex and time consuming. This work reports the results of a real-time PCR assay (TaqMan“) for detecting phytoplasmas in grapevines affected by FD. We obtained specific detection in samples from seven FD-affected grapevines. This method gave no signal with phytoplasmas associated with another grapevine disease (Bois noir) or with eleven reference phytoplasma strains grown in periwinkle. We propose Real-time PCR as an alternative and rapid method for the detection of phytoplasmas in grapevine.
Detection of phytoplasmas associated with grapevine Flavescence dorée disease using real-time PCR / P.A. Bianco, P. Casati, N. Marziliano. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - 86:3(2004), pp. 257-261.
Detection of phytoplasmas associated with grapevine Flavescence dorée disease using real-time PCR
P.A. BiancoPrimo
;P. CasatiSecondo
;
2004
Abstract
Flavescence dorée (FD) of grapevine is a serious disease caused by phytoplasmas. Currently available protocols for phytoplasma detection in grapevine are complex and time consuming. This work reports the results of a real-time PCR assay (TaqMan“) for detecting phytoplasmas in grapevines affected by FD. We obtained specific detection in samples from seven FD-affected grapevines. This method gave no signal with phytoplasmas associated with another grapevine disease (Bois noir) or with eleven reference phytoplasma strains grown in periwinkle. We propose Real-time PCR as an alternative and rapid method for the detection of phytoplasmas in grapevine.Pubblicazioni consigliate
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