Currently, assessment of bacterial biofilm formation using crystal-violet microtiter plate assay presents challenges in both method consistency and underlying biological rationale. This problem has limited comparison of results among researchers and standardized practical interpretation as a virulence factor. We propose a Standardized Biofilm Ratio (SBR) calculation model protocol for in vitro biofilm evaluation in Gram-negative bacteria.
Standardized biofilm ratio: a comparative tool for crystal-violet microtiter plate assay testing in gram-negative bacteria / M.A. Munoz, M.V.D.S.. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - 250:(2026 Nov), pp. 107701.1-107701.3. [10.1016/j.mimet.2026.107701]
Standardized biofilm ratio: a comparative tool for crystal-violet microtiter plate assay testing in gram-negative bacteria
P. Moroni;
2026
Abstract
Currently, assessment of bacterial biofilm formation using crystal-violet microtiter plate assay presents challenges in both method consistency and underlying biological rationale. This problem has limited comparison of results among researchers and standardized practical interpretation as a virulence factor. We propose a Standardized Biofilm Ratio (SBR) calculation model protocol for in vitro biofilm evaluation in Gram-negative bacteria.
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