Reliable, and easy to measure, immunological markers able to denote disease characteristics in multiple sclerosis (MS) patients are still lacking. We applied a multivariate statistical analysis on results obtained by measuring-by real-time RT-PCR-mRNA levels of 25 immunological relevant molecules in PBMCs from 198 MS patients. The combined measurement of mRNA levels of IL-1(beta), TNF-(alpha), TGF-(beta), CCL20 and CCR3 was able to distinguish MS patients from healthy individuals. CXCR5, CCL5, and CCR3 combined mRNA levels identify primary progressive MS patients while TNF-(alpha), IL-10, CXCL10 and CCR3 differentiate relapsing MS patients. Our results indicate that multi-parametric analysis of mRNA levels of immunological relevant molecules in PBMCs may represent a successful strategy for the identification of putative peripheral markers of disease state and disease activity in MS patients.
|Titolo:||Immunological patterns identifying disease course and evolution in multiple sclerosis patients|
|Autori interni:||SCARPINI, ELIO ANGELO|
|Parole Chiave:||RNA analysis ; adolescent ; adult ; aged ; analytic method ; article ; controlled study ; disease activity ; disease course ; female ; human cell ; human ; major clinical study ; male ; measurement ; molecule ; multiple sclerosis ; multivariate analysis ; patient ; peripheral blood mononuclear cell ; priority journal ; real time polymerase chain reaction ; recurrent disease ; reverse transcription polymerase chain reaction ; statistical analysis ; RANTES ; chemokine receptor CCR3 ; chemokine receptor CXCR5 ; chemokine receptor ; chemokine ; cytokine ; gamma interferon inducible protein 10 ; interleukin 10 ; interleukin 1beta ; macrophage inflammatory protein 1alpha ; macrophage inflammatory protein 3alpha ; messenger RNA ; transforming growth factor beta ; tumor necrosis factor alpha|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||10.1016/j.jneuroim.2005.04.012|
|Appare nelle tipologie:||01 - Articolo su periodico|