Reliable, and easy to measure, immunological markers able to denote disease characteristics in multiple sclerosis (MS) patients are still lacking. We applied a multivariate statistical analysis on results obtained by measuring-by real-time RT-PCR-mRNA levels of 25 immunological relevant molecules in PBMCs from 198 MS patients. The combined measurement of mRNA levels of IL-1(beta), TNF-(alpha), TGF-(beta), CCL20 and CCR3 was able to distinguish MS patients from healthy individuals. CXCR5, CCL5, and CCR3 combined mRNA levels identify primary progressive MS patients while TNF-(alpha), IL-10, CXCL10 and CCR3 differentiate relapsing MS patients. Our results indicate that multi-parametric analysis of mRNA levels of immunological relevant molecules in PBMCs may represent a successful strategy for the identification of putative peripheral markers of disease state and disease activity in MS patients.
Immunological patterns identifying disease course and evolution in multiple sclerosis patients / A. Kurne, G. Comi, A. Bergami, F.M. Boneschi, M. Zaffaroni, A. Uccelli, E. Scarpini, D. Franciotta, F. Agosta, M. Gironi, M. Khademi, R. Furlan, M. Filippi, G. Martino, M. Rovaris, M. Deleidi, T. Olsson. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 165:1-2(2005), pp. 192-200.
Immunological patterns identifying disease course and evolution in multiple sclerosis patients
E. Scarpini;R. Furlan;
2005
Abstract
Reliable, and easy to measure, immunological markers able to denote disease characteristics in multiple sclerosis (MS) patients are still lacking. We applied a multivariate statistical analysis on results obtained by measuring-by real-time RT-PCR-mRNA levels of 25 immunological relevant molecules in PBMCs from 198 MS patients. The combined measurement of mRNA levels of IL-1(beta), TNF-(alpha), TGF-(beta), CCL20 and CCR3 was able to distinguish MS patients from healthy individuals. CXCR5, CCL5, and CCR3 combined mRNA levels identify primary progressive MS patients while TNF-(alpha), IL-10, CXCL10 and CCR3 differentiate relapsing MS patients. Our results indicate that multi-parametric analysis of mRNA levels of immunological relevant molecules in PBMCs may represent a successful strategy for the identification of putative peripheral markers of disease state and disease activity in MS patients.Pubblicazioni consigliate
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