Method for obtaining tumor-hypoxia educated regenerative macrophages and use thereof in regenerative medicine

The present invention relates to an in vitro or ex vivo method for inducing a phenotypic and/or functional change ni a population of mononuclear phagocytes isolated from biological samples comprising the incubation of said population in a culture medium comprising factors released from tumor cultures or explants, wherein the incubation takes place under hypoxic conditions and wherein said incubation induces a phenotypic and / or functional change in the mononuclear phagocytes of the population. The macrophages thus obtained asume aunique regenerative phenotype not present ni other polarized phenotypes, including M2 macrophages. In addition, the invention relates to the use of the bioreactor thus produced for the regeneration of tissues, including neural tissue.