Assessing total magnesium (Mg) becomes challenging when cell quantities are minimal. Here, we present a protocol to quantify total intracellular Mg in samples with as few as several thousand cells using a fluorescent plate reader and the fluorescent dye diaza-18-crown-6-hydroxyquinoline-5 (DCHQ5). We describe steps for reagent setup, sample preparation, and assay running. We then detail procedures for sample analysis. This protocol has been validated on different cell lines with data from the literature.
Protocol to quantify total intracellular magnesium in small samples using a fluorescent plate reader and fluorescent dye / L. Anconelli, G. Farruggia, M. Lombardo, F. Rossi, M.M. Rydzyk, M. Rossi, E. Malucelli, C. Cappadone, J.A. Maier, S. Iotti. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 6:3(2025 Sep), pp. 103956.1-103956.16. [10.1016/j.xpro.2025.103956]
Protocol to quantify total intracellular magnesium in small samples using a fluorescent plate reader and fluorescent dye
J.A. MaierPenultimo
;
2025
Abstract
Assessing total magnesium (Mg) becomes challenging when cell quantities are minimal. Here, we present a protocol to quantify total intracellular Mg in samples with as few as several thousand cells using a fluorescent plate reader and the fluorescent dye diaza-18-crown-6-hydroxyquinoline-5 (DCHQ5). We describe steps for reagent setup, sample preparation, and assay running. We then detail procedures for sample analysis. This protocol has been validated on different cell lines with data from the literature.
| File | Dimensione | Formato | |
|---|---|---|---|
|
star protocol.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Licenza:
Creative commons
Dimensione
5.64 MB
Formato
Adobe PDF
|
5.64 MB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
