CUT&Tag is a method to map the genome-wide distribution of histone modifications and some chromatin-associated proteins. CUT&Tag relies on antibody-targeted chromatin tagmentation and can easily be scaled up or automatized. This protocol provides clear experimental guidelines and helpful considerations when planning and executing CUT&Tag experiments.
Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos / F. Zenk, F. Cardamone, D.A. Ibarra Morales, N. Atinbayeva, Y. Zhan, N. Iovino (METHODS IN MOLECULAR BIOLOGY). - In: Polycomb Group Proteins / [a cura di] C. Lanzuolo, F. Marasca. - [s.l] : Humana Press Inc., 2023. - ISBN 9781071631423. - pp. 1-17 [10.1007/978-1-0716-3143-0_1]
Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos
Y. Zhan
;
2023
Abstract
CUT&Tag is a method to map the genome-wide distribution of histone modifications and some chromatin-associated proteins. CUT&Tag relies on antibody-targeted chromatin tagmentation and can easily be scaled up or automatized. This protocol provides clear experimental guidelines and helpful considerations when planning and executing CUT&Tag experiments.
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