This study examined the in vitro antiangiogenic effects of the somatostatin analog octreotide on the growth of human HUV-EC-C endothelial cells and vascular cells from explants of rat aorta cultured on fibronectin-coated dishes or included in fibrin gel. A total 10(-9) mol/L octreotide reduced the mean uptake of 3H-thymidine by HUV-EC-C cells by 37% compared with controls. The 10(-8) mol/L concentration of octreotide inhibited the proliferation of endothelial and smooth muscle cells growing on fibronectin by 32.6% and reduced the sprouting of cells from the adventitia of aortic rings in fibrin by 33.2% compared with controls, as measured by tetrazolium bioreduction and image analysis, respectively. These results demonstrate that octreotide is an effective inhibitor of vascular cell proliferation in vitro.
This study examined the in vitro antiangiogenic effects of the somatostatin analog octreotide on the growth of human HUV-EC-C endothelial cells and vascular cells from explants of rat aorta cultured on fibronectin-coated dishes or included in fibrin gel. A total 10-9 mol/L octreotide reduced the mean uptake of 3H-thymidine by HUV-EC-C cells by 37% compared with controls. The 10-8 mol/L concentration of octreotide inhibited the proliferation of endothelial and smooth muscle cells growing on fibronectin by 32.6% and reduced the sprouting of cells from the adventitia of aortic rings in fibrin by 33.2% compared with controls, as measured by tetrazolium bioreduction and image analysis, respectively. These results demonstrate that octreotide is an effective inhibitor of vascular cell proliferation in vitro. Copyright © 1996 by W.B. Saunders Company.
The effects of the somatostatin analog octreotide on angiogenesis in vitro / R. Danesi, M. DEL TACCA. - In: METABOLISM, CLINICAL AND EXPERIMENTAL. - ISSN 0026-0495. - 45:8 Suppl 1(1996), pp. 49-50. [10.1016/S0026-0495(96)90080-3]
The effects of the somatostatin analog octreotide on angiogenesis in vitro
R. Danesi;
1996
Abstract
This study examined the in vitro antiangiogenic effects of the somatostatin analog octreotide on the growth of human HUV-EC-C endothelial cells and vascular cells from explants of rat aorta cultured on fibronectin-coated dishes or included in fibrin gel. A total 10-9 mol/L octreotide reduced the mean uptake of 3H-thymidine by HUV-EC-C cells by 37% compared with controls. The 10-8 mol/L concentration of octreotide inhibited the proliferation of endothelial and smooth muscle cells growing on fibronectin by 32.6% and reduced the sprouting of cells from the adventitia of aortic rings in fibrin by 33.2% compared with controls, as measured by tetrazolium bioreduction and image analysis, respectively. These results demonstrate that octreotide is an effective inhibitor of vascular cell proliferation in vitro. Copyright © 1996 by W.B. Saunders Company.Pubblicazioni consigliate
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