The CA.ME.LI.A (CArdiovascular risks, MEtabolic syndrome, LIver, and Autoimmune disease) epidemiological study aimed at exploring the associations between cardiovascular, metabolic, hepatobiliary, and autoimmune diseases in a representative population from Northern Italy (n=2545, 1251 men), with the primary goal of identifying risk factors for cardiovascular disease. In this work, we examined whether a unique sphingolipid profile, potentially a new biomarker for diabesity, could be associated with overweight/obesity and glucose intolerance/diabetes. A random sample of 368 individuals (n=367, 217 men, 150 women) underwent plasma lipid extraction and LC-MS/MS analysis and was stratified according to BMI (NBW < 25 kg/m2, OWO ≥ 25 kg/m2) and fasting glycemia (NFG <100 mg/dl, IFG 100 – 125 mg/dL, DM ≥ 126 mg/dL). By combining these two variables, six groups were created: 1. NFG/NBW; 2. NFG/OWO; 3. IFG/NBW; 4. IFG/OWO; 5. DM/NBW; 6. DM/OWO. The most notable changes were observed when glucose intolerance occurred alongside overweight or obese individuals. Specifically, dihydroceramides were found to be higher in the IFG/OWO group (0.39±0.18 uM) compared to NGT/OWO (0.35±0.18 uM) and DM/OWO (0.32±0.2 uM), suggesting that the accumulation of these molecules may serve as an early marker of glucose intolerance. In diabetic patients, both those with obesity (DM/OWO, 13.9±7.2 uM) and those of normal weight (DM/NBW, 16.6±6 uM), hexosylceramides were significantly lower compared to the other groups (NGT/NBW, 19.9±6 uM). Foremost, sphingosine-1-phosphate, a biologically active sphingolipid with a remarkable signaling activity, was significantly reduced in IFG/OWO (1.8±0.5 uM) and DM/OWO (1.8±0.7 uM) patients compared to NGT/OWO (2.2±0.7 uM). This study highlights notable shifts in plasma sphingolipid levels among individuals with diabetes and glucose intolerance, highlighting their role as promising biomarkers, particularly when glucose intolerance coincides with overweight or obesity, suggesting a potential synergistic effect. Moreover, these alterations may be identifiable even at glucose levels as low as ≥126 mg/dL (7.0 mmol/L), underscoring their diagnostic potential.

The Relationship of Glucose Intolerance/Diabetes and Overweight/Obesity with Sphingolipidoma Alterations / C. Morano, L. Centofanti, M.V. DEI CAS, S. Penati, E. Bianco, M. Bignotto, C. Berra, P. Zermiani, R. Paroni, P.M. Battezzati, F. Folli. ((Intervento presentato al 15. convegno XV Sphingolipid Club Meeting : September, 3 - 7 tenutosi a Erlangen nel 2024.

The Relationship of Glucose Intolerance/Diabetes and Overweight/Obesity with Sphingolipidoma Alterations

C. Morano
Primo
;
L. Centofanti
Secondo
;
M.V. DEI CAS;S. Penati;M. Bignotto;P. Zermiani;R. Paroni;P.M. Battezzati
Penultimo
;
F. Folli
Ultimo
2024

Abstract

The CA.ME.LI.A (CArdiovascular risks, MEtabolic syndrome, LIver, and Autoimmune disease) epidemiological study aimed at exploring the associations between cardiovascular, metabolic, hepatobiliary, and autoimmune diseases in a representative population from Northern Italy (n=2545, 1251 men), with the primary goal of identifying risk factors for cardiovascular disease. In this work, we examined whether a unique sphingolipid profile, potentially a new biomarker for diabesity, could be associated with overweight/obesity and glucose intolerance/diabetes. A random sample of 368 individuals (n=367, 217 men, 150 women) underwent plasma lipid extraction and LC-MS/MS analysis and was stratified according to BMI (NBW < 25 kg/m2, OWO ≥ 25 kg/m2) and fasting glycemia (NFG <100 mg/dl, IFG 100 – 125 mg/dL, DM ≥ 126 mg/dL). By combining these two variables, six groups were created: 1. NFG/NBW; 2. NFG/OWO; 3. IFG/NBW; 4. IFG/OWO; 5. DM/NBW; 6. DM/OWO. The most notable changes were observed when glucose intolerance occurred alongside overweight or obese individuals. Specifically, dihydroceramides were found to be higher in the IFG/OWO group (0.39±0.18 uM) compared to NGT/OWO (0.35±0.18 uM) and DM/OWO (0.32±0.2 uM), suggesting that the accumulation of these molecules may serve as an early marker of glucose intolerance. In diabetic patients, both those with obesity (DM/OWO, 13.9±7.2 uM) and those of normal weight (DM/NBW, 16.6±6 uM), hexosylceramides were significantly lower compared to the other groups (NGT/NBW, 19.9±6 uM). Foremost, sphingosine-1-phosphate, a biologically active sphingolipid with a remarkable signaling activity, was significantly reduced in IFG/OWO (1.8±0.5 uM) and DM/OWO (1.8±0.7 uM) patients compared to NGT/OWO (2.2±0.7 uM). This study highlights notable shifts in plasma sphingolipid levels among individuals with diabetes and glucose intolerance, highlighting their role as promising biomarkers, particularly when glucose intolerance coincides with overweight or obesity, suggesting a potential synergistic effect. Moreover, these alterations may be identifiable even at glucose levels as low as ≥126 mg/dL (7.0 mmol/L), underscoring their diagnostic potential.
set-2024
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore MED/13 - Endocrinologia
Settore BIO/10 - Biochimica
Settore MEDS-10/A - Gastroenterologia
Settore BIOS-09/A - Biochimica clinica e biologia molecolare clinica
The Relationship of Glucose Intolerance/Diabetes and Overweight/Obesity with Sphingolipidoma Alterations / C. Morano, L. Centofanti, M.V. DEI CAS, S. Penati, E. Bianco, M. Bignotto, C. Berra, P. Zermiani, R. Paroni, P.M. Battezzati, F. Folli. ((Intervento presentato al 15. convegno XV Sphingolipid Club Meeting : September, 3 - 7 tenutosi a Erlangen nel 2024.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1096615
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