A 39-member library of bile acid derivatives was prepared starting from 3a,7a,12a-trihydroxy-5b-cholan-24-oic acid methyl ester using a combinatorial biocatalytic approach. A regioselective oxidation step, catalyzed by hydroxysteroid dehydrogenases, followed by an acylation step with a series of different acyl donors catalyzed by Candida antarctica lipase B, led to the modification of the bile acid scaffold. Each member of the library was obtained in high purity and good yield.
|Titolo:||A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives|
|Parole Chiave:||Bile acids; Candida antarctica lipase B; Combinatorial biocatalysis; Hydroxysteroid dehydrogenases|
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
|Data di pubblicazione:||20-feb-2003|
|Digital Object Identifier (DOI):||10.1002/bit.10486|
|Appare nelle tipologie:||01 - Articolo su periodico|