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A Solanum lycopersicum polyamine oxidase contributes to the control of plant growth, xylem differentiation, and drought stress tolerance / R. D'Incà, R. Mattioli, M. Tomasella, R. Tavazza, A. Macone, A. Incocciati, D. Martignago, F. Polticelli, I. Fraudentali, A. Cona, R. Angelini, M. Tavazza, A. Nardini, P. Tavladoraki. - In: PLANT JOURNAL. - ISSN 0960-7412. - (2024), pp. 1-22. [10.1111/tpj.16809]

A Solanum lycopersicum polyamine oxidase contributes to the control of plant growth, xylem differentiation, and drought stress tolerance

D. Martignago;
2024
Polyamines are involved in several plant physiological processes. In Arabidopsis thaliana, five FAD-dependent polyamine oxidases (AtPAO1 to AtPAO5) contribute to polyamine homeostasis. AtPAO5 catalyzes the back-conversion of thermospermine (T-Spm) to spermidine and plays a role in plant development, xylem differentiation, and abiotic stress tolerance. In the present study, to verify whether T-Spm metabolism can be exploited as a new route to improve stress tolerance in crops and to investigate the underlying mechanisms, tomato (Solanum lycopersicum) AtPAO5 homologs were identified (SlPAO2, SlPAO3, and SlPAO4) and CRISPR/Cas9-mediated loss-of-function slpao3 mutants were obtained. Morphological, molecular, and physiological analyses showed that slpao3 mutants display increased T-Spm levels and exhibit changes in growth parameters, number and size of xylem elements, and expression levels of auxin- and gibberellin-related genes compared to wild-type plants. The slpao3 mutants are also characterized by improved tolerance to drought stress, which can be attributed to a diminished xylem hydraulic conductivity that limits water loss, as well as to a reduced vulnerability to embolism. Altogether, this study evidences conservation, though with some significant variations, of the T-Spm-mediated regulatory mechanisms controlling plant growth and differentiation across different plant species and highlights the T-Spm role in improving stress tolerance while not constraining growth.
Solanum lycopersicum; Thermospermine; drought stress tolerance; genome editing for crop improvement; leaf transpiration rate; polyamine oxidase; relative water content; xylem hydraulic conductivity
Settore AGR/07 - Genetica Agraria
Settore BIO/01 - Botanica Generale
Settore BIO/04 - Fisiologia Vegetale
Settore BIO/18 - Genetica
2024
18-mag-2024
PLANT JOURNAL
Article (author)
01 - Articolo su periodico
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1067270
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