Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.

Codon bias can determine sorting of a potassium channel protein / A.J. Engel, M. Kithil, M. Langhans, O. Rauh, M. Cartolano, J.L. Van Etten, A. Moroni, G. Thiel. - In: CELLS. - ISSN 2073-4409. - 10:5(2021 May), pp. 1128.1-1128.17. [10.3390/cells10051128]

Codon bias can determine sorting of a potassium channel protein

A. Moroni
Penultimo
Conceptualization
;
2021

Abstract

Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.
codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting
Settore BIO/09 - Fisiologia
   Noninvasive Manipulation of Gating in Ion Channels
   noMAGIC
   EUROPEAN COMMISSION
   H2020
   695078
mag-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1049215
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