A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion.
Bioencapsulation of Oocytes and Granulosa Cells / M. Faustini, S. Agradi, D. Vigo, M.L. Torre, G. Curone (METHODS IN MOLECULAR BIOLOGY). - In: Epithelial Cell Culture / [a cura di] M. Baratta. - [s.l] : Springer, 2024. - ISBN 9781071636084. - pp. 103-108 [10.1007/978-1-0716-3609-1_10]
Bioencapsulation of Oocytes and Granulosa Cells
M. FaustiniPrimo
;S. AgradiSecondo
;D. Vigo
;G. CuroneUltimo
2024
Abstract
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion.
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