A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion.

Bioencapsulation of Oocytes and Granulosa Cells / M. Faustini, S. Agradi, D. Vigo, M.L. Torre, G. Curone (METHODS IN MOLECULAR BIOLOGY). - In: Epithelial Cell Culture / [a cura di] M. Baratta. - [s.l] : Springer, 2024. - ISBN 9781071636084. - pp. 103-108 [10.1007/978-1-0716-3609-1_10]

Bioencapsulation of Oocytes and Granulosa Cells

M. Faustini
Primo
;
S. Agradi
Secondo
;
D. Vigo
;
G. Curone
Ultimo
2024

Abstract

A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion.
3D culture; Alginate; Encapsulation; Granulosa cells
Settore VET/02 - Fisiologia Veterinaria
2024
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1047999
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