mmune cell phenotyping frequently detects lineage-unrelated receptors. Here, we report that surface receptors can be transferred from primary macrophages to CD4 T cells and identify the Fcγ receptor CD32 as driver and cargo of this trogocytotic transfer. Filamentous CD32+ nanoprotrusions deposit distinct plasma membrane patches onto target T cells. Transferred receptors confer cell migration and adhesion properties, and macrophage-derived membrane patches render resting CD4 T cells susceptible to infection by serving as hotspots for HIV-1 binding. Antibodies that recognize T cell epitopes enhance CD32-mediated trogocytosis. Such autoreactive anti-HIV-1 envelope antibodies can be found in the blood of HIV-1 patients and, consistently, the percentage of CD32+ CD4 T cells is increased in their blood. This CD32-mediated, antigen-independent cell communication mode transiently expands the receptor repertoire and functionality of immune cells. HIV-1 hijacks this mechanism by triggering the generation of trogocytosis-promoting autoantibodies to gain access to immune cells critical to its persistence.
Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells / M. Albanese, H. Chen, M. Gapp, M. Muenchhoff, H. Yang, D. Peterhoff, K. Hoffmann, Q. Xiao, A. Ruhle, I. Ambiel, S. Schneider, E. Mejías-Pérez, M. Stern, P.R. Wratil, K. Hofmann, L. Amann, L. Jocham, T. Fuchs, A.F. Ulivi, S. Besson-Girard, S. Weidlich, J. Schneider, C.D. Spinner, K. Sutter, U. Dittmer, A. Humpe, P. Baumeister, A. Wieser, S. Rothenfusser, J. Bogner, J. Roider, P. Knolle, H. Hengel, R. Wagner, V. Laketa, O.T. Fackler, O.T. Keppler. - In: CELL REPORTS MEDICINE. - ISSN 2666-3791. - 5:4(2024 Apr 16), pp. 101483.1-101483.28. [10.1016/j.xcrm.2024.101483]
Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells
M. AlbanesePrimo
;A.F. Ulivi;
2024
Abstract
mmune cell phenotyping frequently detects lineage-unrelated receptors. Here, we report that surface receptors can be transferred from primary macrophages to CD4 T cells and identify the Fcγ receptor CD32 as driver and cargo of this trogocytotic transfer. Filamentous CD32+ nanoprotrusions deposit distinct plasma membrane patches onto target T cells. Transferred receptors confer cell migration and adhesion properties, and macrophage-derived membrane patches render resting CD4 T cells susceptible to infection by serving as hotspots for HIV-1 binding. Antibodies that recognize T cell epitopes enhance CD32-mediated trogocytosis. Such autoreactive anti-HIV-1 envelope antibodies can be found in the blood of HIV-1 patients and, consistently, the percentage of CD32+ CD4 T cells is increased in their blood. This CD32-mediated, antigen-independent cell communication mode transiently expands the receptor repertoire and functionality of immune cells. HIV-1 hijacks this mechanism by triggering the generation of trogocytosis-promoting autoantibodies to gain access to immune cells critical to its persistence.File | Dimensione | Formato | |
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