γδ T cells provide rapid cellular immunity against pathogens. Here, we conducted matched single-cell RNA-sequencing and γδ-TCR-sequencing to delineate the molecular changes in γδ T cells during a longitudinal study following mRNA SARS-CoV-2 vaccination. While the first dose of vaccine primes Vδ2 T cells, it is the second administration that significantly boosts their immune response. Specifically, the second vaccination uncovers memory features of Vδ2 T cells, shaped by the induction of AP-1 family transcription factors and characterized by a convergent central memory signature, clonal expansion, and an enhanced effector potential. This temporally distinct effector response of Vδ2 T cells was also confirmed in vitro upon stimulation with SARS-CoV-2 spike-peptides. Indeed, the second challenge triggers a significantly higher production of IFNγ by Vδ2 T cells. Collectively, our findings suggest that mRNA SARS-CoV-2 vaccination might benefit from the establishment of long- lasting central memory Vδ2 T cells to confer protection against SARS-CoV-2 infection.

Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics / S. Terzoli, P. Marzano, V. Cazzetta, R. Piazza, I. Sandrock, S. Ravens, L. Tan, I. Prinz, S. Balin, M. Calvi, A. Carletti, A. Cancellara, N. Coianiz, S. Franzese, A. Frigo, A. Voza, F. Calcaterra, C. DI VITO, S.A.M. DELLA BELLA, J. Mikulak, D. Mavilio. - In: NPJ VACCINES. - ISSN 2059-0105. - 9:1(2024 Mar 20), pp. 63.1-63.16. [10.1038/s41541-024-00853-9]

Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics

P. Marzano
Secondo
;
V. Cazzetta;S. Balin;M. Calvi;A. Cancellara;S. Franzese;A. Frigo;F. Calcaterra;C. DI VITO;S.A.M. DELLA BELLA
Penultimo
;
J. Mikulak
Co-ultimo
;
D. Mavilio
Co-ultimo
2024

Abstract

γδ T cells provide rapid cellular immunity against pathogens. Here, we conducted matched single-cell RNA-sequencing and γδ-TCR-sequencing to delineate the molecular changes in γδ T cells during a longitudinal study following mRNA SARS-CoV-2 vaccination. While the first dose of vaccine primes Vδ2 T cells, it is the second administration that significantly boosts their immune response. Specifically, the second vaccination uncovers memory features of Vδ2 T cells, shaped by the induction of AP-1 family transcription factors and characterized by a convergent central memory signature, clonal expansion, and an enhanced effector potential. This temporally distinct effector response of Vδ2 T cells was also confirmed in vitro upon stimulation with SARS-CoV-2 spike-peptides. Indeed, the second challenge triggers a significantly higher production of IFNγ by Vδ2 T cells. Collectively, our findings suggest that mRNA SARS-CoV-2 vaccination might benefit from the establishment of long- lasting central memory Vδ2 T cells to confer protection against SARS-CoV-2 infection.
Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio
Settore MED/04 - Patologia Generale
Settore MED/50 - Scienze Tecniche Mediche Applicate
Settore MED/15 - Malattie del Sangue
20-mar-2024
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1041698
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