The immune system in insects is innate and relies both on humoral components and cellular response, which includes phagocytosis, nodulation, and encapsulation. Humoral and cellular defenses are inextricably linked in a complex immune reaction, modulated by a number of cross-talking pathways, which can result in a remarkable variability of any measurement. Protocols to assess the immune response are quite critical and should be carefully standardized, in order to achieve a desirable level of reproducibility. Here we report methods for measuring the cellular immune response in insects, using lepidopteran larvae as model. These protocols have been developed starting from the fragmented information available in the literature, which has been integrated and refined on the basis of our experience on the study of insect immunosuppression by parasitoids and pathogens.

Analysis of Cellular Immune Responses in Lepidopteran Larvae / A. Becchimanzi, I. Di Lelio, F. Pennacchio, S. Caccia (SPRINGER PROTOCOLS). - In: Immunity in Insects / [a cura di] F. Sandrelli, G. Tettamanti. - 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA : HUMANA PRESS INC, 2020. - ISBN 9781071602584. - pp. 97-111

Analysis of Cellular Immune Responses in Lepidopteran Larvae

S. Caccia
Ultimo
2020

Abstract

The immune system in insects is innate and relies both on humoral components and cellular response, which includes phagocytosis, nodulation, and encapsulation. Humoral and cellular defenses are inextricably linked in a complex immune reaction, modulated by a number of cross-talking pathways, which can result in a remarkable variability of any measurement. Protocols to assess the immune response are quite critical and should be carefully standardized, in order to achieve a desirable level of reproducibility. Here we report methods for measuring the cellular immune response in insects, using lepidopteran larvae as model. These protocols have been developed starting from the fragmented information available in the literature, which has been integrated and refined on the basis of our experience on the study of insect immunosuppression by parasitoids and pathogens.
Insect immunity; Hemocytes; Phagocytosis; Nodulation; Encapsulation;
Settore AGR/11 - Entomologia Generale e Applicata
Settore BIO/05 - Zoologia
2020
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1039951
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