Glycolipids have a considerable influence on the interaction between adjacent biomembranes and can promote membrane adhesion trough favorable sugar-sugar "bonds" even at low glycolipid fractions. Here, in order to obtain structural insights into this phenomenon, we utilize neutron reflectometry in combination with a floating lipid bilayer architecture that brings two glycolipid-loaded lipid bilayers to close proximity. We find that selected glycolipids with di-, or oligosaccharide headgroups affect the inter-bilayer water layer thickness and appear to contribute to the stability of the double-bilayer architecture by promoting adhesion of adjacent bilayers even against induced electrostatic repulsion. However, we do not observe any redistribution of glycolipids that would maximize the density of sugar-sugar contacts. Our results point towards possible strategies for the investigation of interactions between cell surfaces involving specific protein-protein, lipid-lipid, or protein-lipid binding.Glycolipids affect the inter-bilayer water layer thickness and contribute to the stability of the double bilayer architecture through the formation of "sugar bonds".

Influence of adhesion-promoting glycolipids on the structure and stability of solid-supported lipid double-bilayers / L. Bange, T. Mukhina, G. Fragneto, V. Rondelli, E. Schneck. - In: SOFT MATTER. - ISSN 1744-6848. - 20:9(2024 Feb 08), pp. 2113-2125. [10.1039/D3SM01615C]

Influence of adhesion-promoting glycolipids on the structure and stability of solid-supported lipid double-bilayers

V. Rondelli
Penultimo
;
2024

Abstract

Glycolipids have a considerable influence on the interaction between adjacent biomembranes and can promote membrane adhesion trough favorable sugar-sugar "bonds" even at low glycolipid fractions. Here, in order to obtain structural insights into this phenomenon, we utilize neutron reflectometry in combination with a floating lipid bilayer architecture that brings two glycolipid-loaded lipid bilayers to close proximity. We find that selected glycolipids with di-, or oligosaccharide headgroups affect the inter-bilayer water layer thickness and appear to contribute to the stability of the double-bilayer architecture by promoting adhesion of adjacent bilayers even against induced electrostatic repulsion. However, we do not observe any redistribution of glycolipids that would maximize the density of sugar-sugar contacts. Our results point towards possible strategies for the investigation of interactions between cell surfaces involving specific protein-protein, lipid-lipid, or protein-lipid binding.Glycolipids affect the inter-bilayer water layer thickness and contribute to the stability of the double bilayer architecture through the formation of "sugar bonds".
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
   Multimodal Innovative THeranostic nanoSystem
   MITHoS
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   2020MHL8S9_002
8-feb-2024
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1039412
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