In gymnosperms such as Ginkgo biloba, the pollen's arrival plays a key role in ovule development, before fertilization occurs. Accordingly, G. biloba female plants geographically isolated from male plants aborted all their ovules after the pollination drop emission, which is the event that allows the ovule to capture pollen grains. To decipher the mechanism induced by pollination required to avoid ovule senescence and then abortion, we compared the transcriptomic of pollinated and unpollinated ovules at three time points after the end of the emission of pollination drops. Transcriptomic and in situ expression analyses revealed that several key genes involved in programmed cell death such as senescence and apoptosis, DNA replication, and cell cycle were differentially expressed in unpollinated ovules compared to pollinated ones. Interestingly, we provided evidence that the pollen captured by the pollination drop affects auxin local accumulation and might cause the deregulation of key genes required for ovule's programmed cell death and activating both the cell cycle and DNA replication genes.

Development of pollinated and unpollinated ovules in Ginkgo biloba: unravelling pollen’s role in ovule tissue maturation / A. Muto, E. Talarico, G. D’Apice, M. Di Marzo, S. Moschin, S. Nigris, N. Babolin, E. Greco, F. Araniti, A. Chiappetta, L. Colombo, B. Baldan, L. Bruno. - In: JOURNAL OF EXPERIMENTAL BOTANY. - ISSN 0022-0957. - (2024). [Epub ahead of print] [10.1093/jxb/erae102]

Development of pollinated and unpollinated ovules in Ginkgo biloba: unravelling pollen’s role in ovule tissue maturation

M. Di Marzo;N. Babolin;F. Araniti;L. Colombo;
2024

Abstract

In gymnosperms such as Ginkgo biloba, the pollen's arrival plays a key role in ovule development, before fertilization occurs. Accordingly, G. biloba female plants geographically isolated from male plants aborted all their ovules after the pollination drop emission, which is the event that allows the ovule to capture pollen grains. To decipher the mechanism induced by pollination required to avoid ovule senescence and then abortion, we compared the transcriptomic of pollinated and unpollinated ovules at three time points after the end of the emission of pollination drops. Transcriptomic and in situ expression analyses revealed that several key genes involved in programmed cell death such as senescence and apoptosis, DNA replication, and cell cycle were differentially expressed in unpollinated ovules compared to pollinated ones. Interestingly, we provided evidence that the pollen captured by the pollination drop affects auxin local accumulation and might cause the deregulation of key genes required for ovule's programmed cell death and activating both the cell cycle and DNA replication genes.
Auxin; Female Gametophyte; Gymnosperm; Ovule; Pollen; Senescence; Transcriptome; Transcription factors
Settore BIO/18 - Genetica
Settore BIO/01 - Botanica Generale
Settore AGR/13 - Chimica Agraria
   Evolution of molecular networks controlling seed coat differentiation
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   20175R447S_01

   Evolution of genetic network required for fruit and fruit-like structures development of land plants (EVOfruland)
   EVOfruland
   EUROPEAN COMMISSION
   H2020
   101007738
2024
9-mar-2024
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1038228
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