The mammalian ovary is a substantial source of oocytes arranged into follicles at various stages of folliculogenesis, from the primordial to the ovulatory ones. Primordial follicles constitute the most abundant source of gametes inside the mammalian ovary at any given time.The isolation of a high number of primordial follicles, together with the development of protocols for in vitro follicle growth, would provide a powerful tool to fully exploit the female reproductive potential and boost the rescue and restoration of fertility in assisted reproduction technologies in human medicine, animal breeding, and preservation of threatened species. However, the most significant limitation is the lack of efficient methods for isolating a healthy and homogeneous population of viable primordial follicles suitable for in vitro culture. Here, we provide a fast and high-yield strategy for the mechanical isolation of primordial follicles from limited portions of the ovarian cortex in the bovine animal model.
Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model / P. Dey, N. Monferini, L. Donadini, V. Lodde, F. Franciosi, A.M. Luciano (METHODS IN MOLECULAR BIOLOGY). - In: Germ Cell Development : Methods and Protocol / [a cura di] M. Barchi, M. De Felici. - Cham : Springer Nature, 2024. - ISBN 9781071636978. - pp. 171-182 [10.1007/978-1-0716-3698-5_13]
Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model
P. DeyPrimo
;N. MonferiniSecondo
;L. Donadini;V. Lodde;F. FranciosiPenultimo
;A.M. Luciano
Ultimo
2024
Abstract
The mammalian ovary is a substantial source of oocytes arranged into follicles at various stages of folliculogenesis, from the primordial to the ovulatory ones. Primordial follicles constitute the most abundant source of gametes inside the mammalian ovary at any given time.The isolation of a high number of primordial follicles, together with the development of protocols for in vitro follicle growth, would provide a powerful tool to fully exploit the female reproductive potential and boost the rescue and restoration of fertility in assisted reproduction technologies in human medicine, animal breeding, and preservation of threatened species. However, the most significant limitation is the lack of efficient methods for isolating a healthy and homogeneous population of viable primordial follicles suitable for in vitro culture. Here, we provide a fast and high-yield strategy for the mechanical isolation of primordial follicles from limited portions of the ovarian cortex in the bovine animal model.File | Dimensione | Formato | |
---|---|---|---|
MiMBv9.0AIR.pdf
accesso aperto
Tipologia:
Pre-print (manoscritto inviato all'editore)
Dimensione
343.08 kB
Formato
Adobe PDF
|
343.08 kB | Adobe PDF | Visualizza/Apri |
978-1-0716-3698-5_13.pdf
accesso riservato
Descrizione: Book Chapter
Tipologia:
Publisher's version/PDF
Dimensione
448.77 kB
Formato
Adobe PDF
|
448.77 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.