The mammalian ovary is a substantial source of oocytes arranged into follicles at various stages of folliculogenesis, from the primordial to the ovulatory ones. Primordial follicles constitute the most abundant source of gametes inside the mammalian ovary at any given time.The isolation of a high number of primordial follicles, together with the development of protocols for in vitro follicle growth, would provide a powerful tool to fully exploit the female reproductive potential and boost the rescue and restoration of fertility in assisted reproduction technologies in human medicine, animal breeding, and preservation of threatened species. However, the most significant limitation is the lack of efficient methods for isolating a healthy and homogeneous population of viable primordial follicles suitable for in vitro culture. Here, we provide a fast and high-yield strategy for the mechanical isolation of primordial follicles from limited portions of the ovarian cortex in the bovine animal model.

Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model / P. Dey, N. Monferini, L. Donadini, V. Lodde, F. Franciosi, A.M. Luciano (METHODS IN MOLECULAR BIOLOGY). - In: Germ Cell Development : Methods and Protocol / [a cura di] M. Barchi, M. De Felici. - Cham : Springer Nature, 2024. - ISBN 9781071636978. - pp. 171-182 [10.1007/978-1-0716-3698-5_13]

Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model

P. Dey
Primo
;
N. Monferini
Secondo
;
L. Donadini;V. Lodde;F. Franciosi
Penultimo
;
A.M. Luciano
Ultimo
2024

Abstract

The mammalian ovary is a substantial source of oocytes arranged into follicles at various stages of folliculogenesis, from the primordial to the ovulatory ones. Primordial follicles constitute the most abundant source of gametes inside the mammalian ovary at any given time.The isolation of a high number of primordial follicles, together with the development of protocols for in vitro follicle growth, would provide a powerful tool to fully exploit the female reproductive potential and boost the rescue and restoration of fertility in assisted reproduction technologies in human medicine, animal breeding, and preservation of threatened species. However, the most significant limitation is the lack of efficient methods for isolating a healthy and homogeneous population of viable primordial follicles suitable for in vitro culture. Here, we provide a fast and high-yield strategy for the mechanical isolation of primordial follicles from limited portions of the ovarian cortex in the bovine animal model.
Culture; Folliculogenesis; In vitro growth; Mechanical isolation; Oocyte; Ovary; Preantral follicle; Primordial follicle; Viability
Settore VET/01 - Anatomia degli Animali Domestici
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
   New perspectives for the exploitation of female reproductive potential in mammals: from the recovery of the untapped natural ovarian reserve to the generation of oocytes and granulosa cells from mesenchymal stem cells.
   InfinitEGG
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   20209L8BN4_001

   European Oocyte Biology Research Innovation Training Net (EUROVA)
   EUROVA
   EUROPEAN COMMISSION
   H2020
   860960
2024
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1030252
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