Detailed procedures are presented for obtaining highly enriched human Daudi cell population in different cell cycle phases. This protocol allows harvest of enriched G1 or S-G2 phase cells based on the recognition of a cell surface marker antigen by anti-CD11a antibody and sorting. Sorted cells are able to cycle and a wave of synchronisation appears. Relative advantages of the method over chemically induced cell synchronisation are discussed.
Sorting of cells from different cell cycle phases using surface antigen expression / F. Reno', L. Francesca, V. Marco, G. Armando, V. Massimo, P. Stefano. - In: METHODS IN CELL SCIENCE. - ISSN 1381-5741. - 18:(1996), pp. 93-98. [10.1007/BF00122159]
Sorting of cells from different cell cycle phases using surface antigen expression
F. Reno'Primo
;
1996
Abstract
Detailed procedures are presented for obtaining highly enriched human Daudi cell population in different cell cycle phases. This protocol allows harvest of enriched G1 or S-G2 phase cells based on the recognition of a cell surface marker antigen by anti-CD11a antibody and sorting. Sorted cells are able to cycle and a wave of synchronisation appears. Relative advantages of the method over chemically induced cell synchronisation are discussed.
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