Objective: To investigate induction of matrix metalloproteinases (MMPs) during mechanical compression of hypertrophic scars. Mechanical pressure blocks hypertrophy inducted on extracellular matrix in scars by a mechanism that involves MMP-2 (gelatinase A) and MMP-9 (gelatinase B). Design: We assayed conditioned media obtained from normotrophic and hypertrophic scars during 24 hours of in vitro mechanical compression using gelatin zymography. Setting: Scars from various areas of the bodies of hospitalized patients. Patients: We obtained 3 normotrophic and 7 hypertrophic biopsy specimens from 10 patients (5 men and 5 women). Intervention: In vitro compression at a pressure of 35 mm Hg/cm2 for 24 hours. Main Outome Measures: Vitality of scars was analyzed by means of lactic dehydrogenase test; medium samples were collected for zymographic analysis of MMP activity. Results: We found MMP-2 in basal (uncompressed) samples from normotrophic and hypertrophic scars. Mechanical compression induced MMP-9 release and activation (range, 86.7%-78.7%) in hypertrophic scars after 4 hours. Conclusion: Production, release, and activation of MMP-9 in hypertrophic scars could be an effector mechanism responsible for hypertrophy regression induced by mechanical compression.

Release and activation of matrix metalloproteinase-9 during in vitro mechanical compression in hypertrophic scars / F. Reno', P. Grazianetti, M. Stella, G. Magliacani, C. Pezzuto, M. Cannas. - In: ARCHIVES OF DERMATOLOGY. - ISSN 0003-987X. - 138:4(2002), pp. 475-478. [10.1001/archderm.138.4.475]

Release and activation of matrix metalloproteinase-9 during in vitro mechanical compression in hypertrophic scars

F. Reno';
2002

Abstract

Objective: To investigate induction of matrix metalloproteinases (MMPs) during mechanical compression of hypertrophic scars. Mechanical pressure blocks hypertrophy inducted on extracellular matrix in scars by a mechanism that involves MMP-2 (gelatinase A) and MMP-9 (gelatinase B). Design: We assayed conditioned media obtained from normotrophic and hypertrophic scars during 24 hours of in vitro mechanical compression using gelatin zymography. Setting: Scars from various areas of the bodies of hospitalized patients. Patients: We obtained 3 normotrophic and 7 hypertrophic biopsy specimens from 10 patients (5 men and 5 women). Intervention: In vitro compression at a pressure of 35 mm Hg/cm2 for 24 hours. Main Outome Measures: Vitality of scars was analyzed by means of lactic dehydrogenase test; medium samples were collected for zymographic analysis of MMP activity. Results: We found MMP-2 in basal (uncompressed) samples from normotrophic and hypertrophic scars. Mechanical compression induced MMP-9 release and activation (range, 86.7%-78.7%) in hypertrophic scars after 4 hours. Conclusion: Production, release, and activation of MMP-9 in hypertrophic scars could be an effector mechanism responsible for hypertrophy regression induced by mechanical compression.
Settore BIO/16 - Anatomia Umana
2002
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1020215
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