Alpha-synuclein (αSyn) is a small presynaptic protein (14 kDa) that is involved in synucleinopathies including Parkinson's disease (PD). In its native state, the αSyn monomer exists in an unfolded state, and its folding is highly dependent on variations of environmental conditions, mutations and interactions with endogenous and/or exogenous molecules. Recently, there is increasing evidence for a direct interplay between αSyn and microtubules (MTs), whose defects are linked to neurodegenerative diseases, such as PD. Understanding the correlation between αSyn and MTs could be fundamental for the correct comprehension of the undergoing mechanisms of PD. Hence, we chemically synthesized a library of peptides, deriving from both native and PD mutated sequences of the N-terminal domain of αSyn. Their secondary structure was characterized by circular dichroism and Fourier transform infrared (FTIR) experiments, in order to evaluate the effect of PD mutations. Finally, the kinetics of polymerizing tubulin in vitro in the presence of the peptides was evaluated.
Breaking down and building up alpha‐synuclein: An insight on its N‐terminal domain / K. Peqini, S. Attanasio, L. Feni, G. Cappelletti, S. Pellegrino. - In: JOURNAL OF PEPTIDE SCIENCE. - ISSN 1075-2617. - (2023), pp. e3556.1-e3556.8. [Epub ahead of print] [10.1002/psc.3556]
Breaking down and building up alpha‐synuclein: An insight on its N‐terminal domain
K. PeqiniPrimo
;S. AttanasioSecondo
;L. Feni;G. CappellettiPenultimo
;S. Pellegrino
Ultimo
2023
Abstract
Alpha-synuclein (αSyn) is a small presynaptic protein (14 kDa) that is involved in synucleinopathies including Parkinson's disease (PD). In its native state, the αSyn monomer exists in an unfolded state, and its folding is highly dependent on variations of environmental conditions, mutations and interactions with endogenous and/or exogenous molecules. Recently, there is increasing evidence for a direct interplay between αSyn and microtubules (MTs), whose defects are linked to neurodegenerative diseases, such as PD. Understanding the correlation between αSyn and MTs could be fundamental for the correct comprehension of the undergoing mechanisms of PD. Hence, we chemically synthesized a library of peptides, deriving from both native and PD mutated sequences of the N-terminal domain of αSyn. Their secondary structure was characterized by circular dichroism and Fourier transform infrared (FTIR) experiments, in order to evaluate the effect of PD mutations. Finally, the kinetics of polymerizing tubulin in vitro in the presence of the peptides was evaluated.File | Dimensione | Formato | |
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Journal of Peptide Science - 2023 - Peqini - Breaking down and building up alpha‐synuclein An insight on its N‐terminal.pdf
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