A Pseudomonas aeruginosa-Suitable Fluorescent Reporter System for Analyzing Small RNA-Mediated Regulation of Target mRNAs

Bacterial small RNAs have emerged as modulators that play key roles in regulatory networks related to viability, environmental adaptation, and pathogenesis. Small RNAs can modulate gene expression by base-pairing to target mRNAs, influencing their translation and/or stability. A superfolder GFP reporter system, previously developed for Escherichia coli and Salmonella enterica, was adapted to Pseudomonas aeruginosa and used to validate novel mRNA targets in studies of small RNA-mediated regulatory mechanisms.