Seven isoforms of 85 kDa polypeptides (p85) were identified as methionine synthase (MetE) homologs by partial aminoacid sequencing in tobacco pollen tube extracts. Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that appear to be partially associated with microtubules (Mts). The chemical dissection of pollen tube high speed supernatant (HSS) showed that two distinct pools of MetE are present in pollen tubes, one being the more acidic isoforms sedimenting at 15S and the remaining at 4S after zonal centrifugation through a sucrose density gradient. The identification of the MetE within the pollen tube and its possible participation as methyl donor in a wide range of metabolic reactions, makes it a good subject for studies on pollen tube growth regulation.

A methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes / A. Moscatelli, M. Scali, C. Prescianotto-Baschong, M. Ferro, J. Garin, R. Vignani, F. Ciampolini, M. Cresti. - In: PLANTA. - ISSN 0032-0935. - 221:6(2005), pp. 776-789.

A methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes

A. Moscatelli
Primo
;
2005

Abstract

Seven isoforms of 85 kDa polypeptides (p85) were identified as methionine synthase (MetE) homologs by partial aminoacid sequencing in tobacco pollen tube extracts. Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that appear to be partially associated with microtubules (Mts). The chemical dissection of pollen tube high speed supernatant (HSS) showed that two distinct pools of MetE are present in pollen tubes, one being the more acidic isoforms sedimenting at 15S and the remaining at 4S after zonal centrifugation through a sucrose density gradient. The identification of the MetE within the pollen tube and its possible participation as methyl donor in a wide range of metabolic reactions, makes it a good subject for studies on pollen tube growth regulation.
methionine synthase; microtubules; Nicotiana tabacum; pollen tube; secretory vesicles
Settore BIO/01 - Botanica Generale
2005
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/9389
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