Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza, A.; Chang, H.-.; Radbruch, A.; Abrignani, S.; Addo, R.; Akdis, M.; Andra, I.; Andreata, F.; Annunziato, F.; Arranz, E.; Bacher, P.; Bari, S.; Barnaba, V.; Barros-Martins, J.; Baumjohann, D.; Beccaria, C.G.; Bernardo, D.; Boardman, D.A.; Borger, J.; Bottcher, C.; Brockmann, L.; Burns, M.; Busch, D.H.; Cameron, G.; Cammarata, I.; Cassotta, A.; Chang, Y.; Chirdo, F.G.; Christakou, E.; Cicin-Sain, L.; Cook, L.; Corbett, A.J.; Cornelis, R.; Cosmi, L.; Davey, M.S.; De Biasi, S.; De Simone, G.; del Zotto, G.; Delacher, M.; Di Rosa, F.; Santo, J.D.; Diefenbach, A.; Dong, J.; Dorner, T.; Dress, R.J.; Dutertre, C.-.; Eckle, S.B.G.; Eede, P.; Evrard, M.; Falk, C.S.; Feuerer, M.; Fillatreau, S.; Fiz-Lopez, A.; Follo, M.; Foulds, G.A.; Frobel, J.; Gagliani, N.; Galletti, G.; Gangaev, A.; Garbi, N.; Garrote, J.A.; Geginat, J.; Gherardin, N.A.; Gibellini, L.; Ginhoux, F.; Godfrey, D.I.; Gruarin, P.; Haftmann, C.; Hansmann, L.; Harpur, C.M.; Hayday, A.C.; Heine, G.; Hernandez, D.C.; Herrmann, M.; Hoelsken, O.; Huang, Q.; Huber, S.; Huber, J.E.; Huehn, J.; Hundemer, M.; Hwang, W.Y.K.; Iannacone, M.; Ivison, S.M.; Jack, H.-.; Jani, P.K.; Keller, B.; Kessler, N.; Ketelaars, S.; Knop, L.; Knopf, J.; Koay, H.-.; Kobow, K.; Kriegsmann, K.; Kristyanto, H.; Krueger, A.; Kuehne, J.F.; Kunze-Schumacher, H.; Kvistborg, P.; Kwok, I.; Latorre, D.; Lenz, D.; Levings, M.K.; Lino, A.C.; Liotta, F.; Long, H.M.; Lugli, E.; Macdonald, K.N.; Maggi, L.; Maini, M.K.; Mair, F.; Manta, C.; Manz, R.A.; Mashreghi, M.-.; Mazzoni, A.; Mccluskey, J.; Mei, H.E.; Melchers, F.; Melzer, S.; Mielenz, D.; Monin, L.; Moretta, L.; Multhoff, G.; Munoz, L.E.; Munoz-Ruiz, M.; Muscate, F.; Natalini, A.; Neumann, K.; L. G., N.; Niedobitek, A.; Niemz, J.; Almeida, L.N.; Notarbartolo, S.; Ostendorf, L.; Pallett, L.J.; Patel, A.A.; Percin, G.I.; Peruzzi, G.; Pinti, M.; Pockley, A.G.; Pracht, K.; Prinz, I.; Pujol-Autonell, I.; Pulvirenti, N.; Quatrini, L.; Quinn, K.M.; Radbruch, H.; Rhys, H.; Rodrigo, M.B.; Romagnani, C.; Saggau, C.; Sakaguchi, S.; Sallusto, F.; Sanderink, L.; Sandrock, I.; Schauer, C.; Scheffold, A.; Scherer, H.U.; Schiemann, M.; Schildberg, F.A.; Schober, K.; Schoen, J.; Schuh, W.; Schuler, T.; Schulz, A.R.; Schulz, S.; Schulze, J.; Simonetti, S.; Singh, J.; Sitnik, K.M.; Stark, R.; Starossom, S.; Stehle, C.; Szelinski, F.; Tan, L.; Tarnok, A.; Tornack, J.; Tree, T.I.M.; van Beek, J.J.P.; van de Veen, W.; van Gisbergen, K.; Vasco, C.; Verheyden, N.A.; von Borstel, A.; Ward-Hartstonge, K.A.; Warnatz, K.; Waskow, C.; Wiedemann, A.; Wilharm, A.; Wing, J.; Wirz, O.; Wittner, J.; Yang, J.H.M.; Yang, J.

doi:10.1002/eji.202170126

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) / A. Cossarizza, H.-. Chang, A. Radbruch, S. Abrignani, R. Addo, M. Akdis, I. Andra, F. Andreata, F. Annunziato, E. Arranz, P. Bacher, S. Bari, V. Barnaba, J. Barros-Martins, D. Baumjohann, C.G. Beccaria, D. Bernardo, D.A. Boardman, J. Borger, C. Bottcher, L. Brockmann, M. Burns, D.H. Busch, G. Cameron, I. Cammarata, A. Cassotta, Y. Chang, F.G. Chirdo, E. Christakou, L. Cicin-Sain, L. Cook, A.J. Corbett, R. Cornelis, L. Cosmi, M.S. Davey, S. De Biasi, G. De Simone, G. del Zotto, M. Delacher, F. Di Rosa, J.D. Santo, A. Diefenbach, J. Dong, T. Dorner, R.J. Dress, C.-. Dutertre, S.B.G. Eckle, P. Eede, M. Evrard, C.S. Falk, M. Feuerer, S. Fillatreau, A. Fiz-Lopez, M. Follo, G.A. Foulds, J. Frobel, N. Gagliani, G. Galletti, A. Gangaev, N. Garbi, J.A. Garrote, J. Geginat, N.A. Gherardin, L. Gibellini, F. Ginhoux, D.I. Godfrey, P. Gruarin, C. Haftmann, L. Hansmann, C.M. Harpur, A.C. Hayday, G. Heine, D.C. Hernandez, M. Herrmann, O. Hoelsken, Q. Huang, S. Huber, J.E. Huber, J. Huehn, M. Hundemer, W.Y.K. Hwang, M. Iannacone, S.M. Ivison, H.-. Jack, P.K. Jani, B. Keller, N. Kessler, S. Ketelaars, L. Knop, J. Knopf, H.-. Koay, K. Kobow, K. Kriegsmann, H. Kristyanto, A. Krueger, J.F. Kuehne, H. Kunze-Schumacher, P. Kvistborg, I. Kwok, D. Latorre, D. Lenz, M.K. Levings, A.C. Lino, F. Liotta, H.M. Long, E. Lugli, K.N. Macdonald, L. Maggi, M.K. Maini, F. Mair, C. Manta, R.A. Manz, M.-. Mashreghi, A. Mazzoni, J. Mccluskey, H.E. Mei, F. Melchers, S. Melzer, D. Mielenz, L. Monin, L. Moretta, G. Multhoff, L.E. Munoz, M. Munoz-Ruiz, F. Muscate, A. Natalini, K. Neumann, L.G. Ng, A. Niedobitek, J. Niemz, L.N. Almeida, S. Notarbartolo, L. Ostendorf, L.J. Pallett, A.A. Patel, G.I. Percin, G. Peruzzi, M. Pinti, A.G. Pockley, K. Pracht, I. Prinz, I. Pujol-Autonell, N. Pulvirenti, L. Quatrini, K.M. Quinn, H. Radbruch, H. Rhys, M.B. Rodrigo, C. Romagnani, C. Saggau, S. Sakaguchi, F. Sallusto, L. Sanderink, I. Sandrock, C. Schauer, A. Scheffold, H.U. Scherer, M. Schiemann, F.A. Schildberg, K. Schober, J. Schoen, W. Schuh, T. Schuler, A.R. Schulz, S. Schulz, J. Schulze, S. Simonetti, J. Singh, K.M. Sitnik, R. Stark, S. Starossom, C. Stehle, F. Szelinski, L. Tan, A. Tarnok, J. Tornack, T.I.M. Tree, J.J.P. van Beek, W. van de Veen, K. van Gisbergen, C. Vasco, N.A. Verheyden, A. von Borstel, K.A. Ward-Hartstonge, K. Warnatz, C. Waskow, A. Wiedemann, A. Wilharm, J. Wing, O. Wirz, J. Wittner, J.H.M. Yang, J. Yang. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - 51:12(2021), pp. 2708-3145. [10.1002/eji.202170126]

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza A.;Chang H. -D.;Radbruch A.;S. Abrignani;Addo R.;Akdis M.;Andra I.;F. Andreata;Annunziato F.;Arranz E.;Bacher P.;Bari S.;Barnaba V.;Barros-Martins J.;Baumjohann D.;Beccaria C. G.;Bernardo D.;Boardman D. A.;Borger J.;Bottcher C.;Brockmann L.;M. Burns;Busch D. H.;Cameron G.;Cammarata I.;Cassotta A.;Chang Y.;Chirdo F. G.;Christakou E.;Cicin-Sain L.;Cook L.;Corbett A. J.;Cornelis R.;Cosmi L.;Davey M. S.;S. De Biasi;De Simone G.;del Zotto G.;Delacher M.;Di Rosa F.;Santo J. D.;Diefenbach A.;Dong J.;Dorner T.;Dress R. J.;Dutertre C. -A.;Eckle S. B. G.;Eede P.;Evrard M.;Falk C. S.;Feuerer M.;Fillatreau S.;Fiz-Lopez A.;Follo M.;Foulds G. A.;Frobel J.;Gagliani N.;Galletti G.;Gangaev A.;Garbi N.;Garrote J. A.;J. Geginat;Gherardin N. A.;Gibellini L.;Ginhoux F.;Godfrey D. I.;Gruarin P.;Haftmann C.;Hansmann L.;Harpur C. M.;Hayday A. C.;Heine G.;Hernandez D. C.;Herrmann M.;Hoelsken O.;Huang Q.;Huber S.;Huber J. E.;Huehn J.;Hundemer M.;Hwang W. Y. K.;Iannacone M.;Ivison S. M.;Jack H. -M.;Jani P. K.;Keller B.;Kessler N.;Ketelaars S.;Knop L.;Knopf J.;Koay H. -F.;Kobow K.;Kriegsmann K.;Kristyanto H.;Krueger A.;Kuehne J. F.;Kunze-Schumacher H.;Kvistborg P.;Kwok I.;D. Latorre;Lenz D.;Levings M. K.;Lino A. C.;Liotta F.;Long H. M.;Lugli E.;MacDonald K. N.;Maggi L.;Maini M. K.;Mair F.;Manta C.;Manz R. A.;Mashreghi M. -F.;A. Mazzoni;McCluskey J.;Mei H. E.;Melchers F.;Melzer S.;Mielenz D.;Monin L.;Moretta L.;Multhoff G.;Munoz L. E.;Munoz-Ruiz M.;Muscate F.;Natalini A.;Neumann K.;Ng L. G.;Niedobitek A.;Niemz J.;Almeida L. N.;S. Notarbartolo;Ostendorf L.;Pallett L. J.;Patel A. A.;Percin G. I.;Peruzzi G.;Pinti M.;Pockley A. G.;Pracht K.;Prinz I.;Pujol-Autonell I.;Pulvirenti N.;Quatrini L.;Quinn K. M.;Radbruch H.;Rhys H.;Rodrigo M. B.;Romagnani C.;Saggau C.;Sakaguchi S.;Sallusto F.;Sanderink L.;Sandrock I.;Schauer C.;Scheffold A.;Scherer H. U.;Schiemann M.;Schildberg F. A.;Schober K.;Schoen J.;Schuh W.;Schuler T.;Schulz A. R.;Schulz S.;Schulze J.;S. Simonetti;Singh J.;Sitnik K. M.;Stark R.;Starossom S.;Stehle C.;Szelinski F.;Tan L.;Tarnok A.;Tornack J.;Tree T. I. M.;van Beek J. J. P.;van de Veen W.;van Gisbergen K.;C. Vasco;Verheyden N. A.;von Borstel A.;Ward-Hartstonge K. A.;Warnatz K.;Waskow C.;Wiedemann A.;Wilharm A.;Wing J.;Wirz O.;Wittner J.;Yang J. H. M.;Yang J.

2021

Abstract

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

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	Settori scientifico-disciplinari dell'articolo
	
			Settore MED/04 - Patologia Generale
		
	Data di pubblicazione
	
			2021
		
	Rivista in ANCE
	
			EUROPEAN JOURNAL OF IMMUNOLOGY
		
	DOI
	
			https://dx.doi.org/10.1002/eji.202170126
		
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			Article (author)
		
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			01 - Articolo su periodico

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