Studies using conventional electron microscopy describe the cytoplasm of lens fiber cells as having essentially an amorphous structure. We hypothesized that significant structural detail might have been lost as a result of projecting the entire thickness of the section (50–100 nm) onto a single plane (the ‘‘projection artifact’’). To test this hypothesis, we studied the 3D-structure of rat lens cortical fibers before and after extracting the ‘‘soluble’’ crystallins with low ionic strength buffers to make ‘‘ghosts.’’ Tomographic series in conical geometry were collected at 55 tilts and by 5 rotations until completing a 360 turn by low dose methods. They were aligned using fiduciary points, reconstructed with the weighted back projection algorithm and refined by projection matching. Analysis of the 3D-maps included semiautomatic density segmentation using a computer program based on the watershed algorithm. We found that the cytoplasm of cortical fibers, though appearing amorphous in regions of the highest density, was in fact comprised of an ordered structure resembling a ‘‘clustered matrix.’’ The matrix was comprised of thin (w6 nm diameter) filaments bent sharply at 110–120 angles and studded with cubeshaped particles (the ‘‘beaded’’ filaments). In cortical fibers, the particles measured a¼ 14 2, b¼ 13 2 and c¼ 10 2.4 nm (n¼ 30, mean SD) and were spaced at distances measuring 27.5 2.4 nm apart (n ¼ 8, mean SD), center-to-center. The matrix was formed as ‘‘beaded’’ filaments, bound to clusters of ‘‘soluble’’ proteins, crossed each other at nearly perpendicular angles. The matrix also made contact with the plasma membrane at a large number of distinct regions. We thus concluded that the cytoplasm of cortical lens fibers is comprised of a cytoskeletal matrix of ‘‘beaded’’ filaments that organize the ‘‘soluble’’ crystallins in separate regions. The association of this matrix with the plasma membrane allows the lens to maintain its structural integrity, while its association with crystallins yields its long-term transparency. Loss of either function likely would play a significant role in cataract formation.

The structure of the cytoplasm of lens fibers as determined by conical tomography / C. Schietroma, N. Fain, L. M. Zampighi, S. Lanzavecchia, G. A. Zampighi. - In: EXPERIMENTAL EYE RESEARCH. - ISSN 0014-4835. - 88:3(2009), pp. 566-574.

The structure of the cytoplasm of lens fibers as determined by conical tomography

S. Lanzavecchia
Penultimo
;
2009

Abstract

Studies using conventional electron microscopy describe the cytoplasm of lens fiber cells as having essentially an amorphous structure. We hypothesized that significant structural detail might have been lost as a result of projecting the entire thickness of the section (50–100 nm) onto a single plane (the ‘‘projection artifact’’). To test this hypothesis, we studied the 3D-structure of rat lens cortical fibers before and after extracting the ‘‘soluble’’ crystallins with low ionic strength buffers to make ‘‘ghosts.’’ Tomographic series in conical geometry were collected at 55 tilts and by 5 rotations until completing a 360 turn by low dose methods. They were aligned using fiduciary points, reconstructed with the weighted back projection algorithm and refined by projection matching. Analysis of the 3D-maps included semiautomatic density segmentation using a computer program based on the watershed algorithm. We found that the cytoplasm of cortical fibers, though appearing amorphous in regions of the highest density, was in fact comprised of an ordered structure resembling a ‘‘clustered matrix.’’ The matrix was comprised of thin (w6 nm diameter) filaments bent sharply at 110–120 angles and studded with cubeshaped particles (the ‘‘beaded’’ filaments). In cortical fibers, the particles measured a¼ 14 2, b¼ 13 2 and c¼ 10 2.4 nm (n¼ 30, mean SD) and were spaced at distances measuring 27.5 2.4 nm apart (n ¼ 8, mean SD), center-to-center. The matrix was formed as ‘‘beaded’’ filaments, bound to clusters of ‘‘soluble’’ proteins, crossed each other at nearly perpendicular angles. The matrix also made contact with the plasma membrane at a large number of distinct regions. We thus concluded that the cytoplasm of cortical lens fibers is comprised of a cytoskeletal matrix of ‘‘beaded’’ filaments that organize the ‘‘soluble’’ crystallins in separate regions. The association of this matrix with the plasma membrane allows the lens to maintain its structural integrity, while its association with crystallins yields its long-term transparency. Loss of either function likely would play a significant role in cataract formation.
electron tomography ; cytoskeleton ; intermediate filaments ; ‘‘beaded’’ filaments ; crystallins ; segmentation
Settore INF/01 - Informatica
2009
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/67205
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