An on-line high-performance liquid immunoaffinity chromatographic (HPLIAC) system for the direct determination of chloramphenicol in milk and swine muscle tissue is described. The system consisted of a dual-column system in which an HPLIAC column was directly coupled to an RP-8 high-performance liquid chromatographic column. Skimmed and deproteinated milk or aqueous muscle tissue extract was directly injected into the HPLIAC column. After a washing step with phosphate-buffered saline, chloramphenicol was desorbed by a glycine-NaCl buffer (pH 2.8) and directly concentrated on the RP-8 column. Next, chromatography was carried out using acetonitrile-sodium acetate buffer as the mobile phase. Chloramphenicol was detected at 280 nm. Mean recoveries from spiked raw milk were 70 +/- 2% (1-50 mug/kg) and from spiked swine muscle tissue 64 +/- 2% (10-50 mug/kg). The calibration curves were linear in the range 1-200 mug/kg spiking levels. Limits of determination were 1 mug/kg for milk and 10 mug/kg for muscle tissue.
Automated high-performance liquid chromatographic determination of chloramphenicol in milk and swine muscle tissue using on-line immunoaffinity sample clean-up / V.M. Moretti, C.van de Water, N. Haagsma. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - 583:1(1992 Nov 27), pp. 77-82.
Automated high-performance liquid chromatographic determination of chloramphenicol in milk and swine muscle tissue using on-line immunoaffinity sample clean-up
V.M. MorettiPrimo
;
1992
Abstract
An on-line high-performance liquid immunoaffinity chromatographic (HPLIAC) system for the direct determination of chloramphenicol in milk and swine muscle tissue is described. The system consisted of a dual-column system in which an HPLIAC column was directly coupled to an RP-8 high-performance liquid chromatographic column. Skimmed and deproteinated milk or aqueous muscle tissue extract was directly injected into the HPLIAC column. After a washing step with phosphate-buffered saline, chloramphenicol was desorbed by a glycine-NaCl buffer (pH 2.8) and directly concentrated on the RP-8 column. Next, chromatography was carried out using acetonitrile-sodium acetate buffer as the mobile phase. Chloramphenicol was detected at 280 nm. Mean recoveries from spiked raw milk were 70 +/- 2% (1-50 mug/kg) and from spiked swine muscle tissue 64 +/- 2% (10-50 mug/kg). The calibration curves were linear in the range 1-200 mug/kg spiking levels. Limits of determination were 1 mug/kg for milk and 10 mug/kg for muscle tissue.Pubblicazioni consigliate
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