Caratterizzazione del ruolo dell'ossido nitrico in un nuovo modello fisiopatologico della sindrome metabolica. Studio di polimorfismi e dell'espressione di geni coinvolti nel metabolismo ossidativo

CARATTERIZZAZIONE DEL RUOLO DELL OSSIDO NITRICO IN UN NUOVO MODELLO FISIOPATOLOGICO DELLA SINDROME METABOLICA STUDIO DI POLIMORFISMI E DELL ESPRESSSIONE DI GENI COINVOLTI NEL METABOLISMO OSSIDATIVO The clinical meaning of the metabolic syndrome, acquires particular importance when typical metabolic disease seem to play a role key in the genesis of the cardiovascular disease and the type 2 diabetes. The metabolic syndrome recognizes multifactorial aetiopathogenesis, with environmental and genetic factors. The first part of the thesis job has the scope of the in vivo characterization of a possible metabolic syndrome animal model. We used knockout for the gene of endothelial nitric oxide synthase (eNOS) mouse and we studied its involvement in the oxidative energy metabolism and in the process of mitochondrial biogenesis. The next step was the study in the obese patients with metabolic syndrome, the role carried out from nitric oxide in the predisposition to such syndrome, by two various approaches. At first a wide population of obese patients was selected, some of which with metabolic syndrome, in order to study the presence of the polymorphism Glu298Asp of the gene of the eNOS, by Real Time PCR. The second approach analyzed the expression of some involved in the oxidative energy metabolism genes from biopsies of subcutaneous human adipose tissue of obese patients with different tolerances to the glucose.