Chlorophenoxy herbicides belong to the peroxisome proliferator (PP) class of non-genotoxic hepatocarcinogens. The ability to stimulate DNA synthesis coupled with the ability to suppress or delay apoptosis may play a role in PP carcinogenesis. There are no conclusive data on the carcinogenicity of phenoxy herbicides. 2,4- dichlorophenoxyacetic acid (2,4-D) is an herbicide widely used in agriculture, forestry, and also in recreational areas. The widespread pattern of 2,4-D use may, therefore, pose a threat to avian organisms as well to other wild species. Primary hepatocyte cultures of chick embryos were used to assess the effects of 2,4-D on S-phase and apoptosis. Considering that PP tumorigenesis is species restricted, the PP Wy-14,643, a rodent hepatocarcinogen, was also tested. Both 2,4-D and Wy-14,643 at a dose level of 100 M induced a slight positive effect on DNA synthesis, as detected after BrdU incorporation, and reduced the number of apoptotic cells. These data support some experimental evidence that 2,4-D may act as a weak tumor promoter, although further experimentation is needed to confirm the results of the present study.
DNA synthesis and apoptosis in primary hepatocyte cultures from chick embryos after treatment with the herbicide 2,4-D / E. Arias. ((Intervento presentato al 17. convegno SETAC Europe Annual Meeting tenutosi a Porto nel 2007.
DNA synthesis and apoptosis in primary hepatocyte cultures from chick embryos after treatment with the herbicide 2,4-D
E. AriasPrimo
2007
Abstract
Chlorophenoxy herbicides belong to the peroxisome proliferator (PP) class of non-genotoxic hepatocarcinogens. The ability to stimulate DNA synthesis coupled with the ability to suppress or delay apoptosis may play a role in PP carcinogenesis. There are no conclusive data on the carcinogenicity of phenoxy herbicides. 2,4- dichlorophenoxyacetic acid (2,4-D) is an herbicide widely used in agriculture, forestry, and also in recreational areas. The widespread pattern of 2,4-D use may, therefore, pose a threat to avian organisms as well to other wild species. Primary hepatocyte cultures of chick embryos were used to assess the effects of 2,4-D on S-phase and apoptosis. Considering that PP tumorigenesis is species restricted, the PP Wy-14,643, a rodent hepatocarcinogen, was also tested. Both 2,4-D and Wy-14,643 at a dose level of 100 M induced a slight positive effect on DNA synthesis, as detected after BrdU incorporation, and reduced the number of apoptotic cells. These data support some experimental evidence that 2,4-D may act as a weak tumor promoter, although further experimentation is needed to confirm the results of the present study.Pubblicazioni consigliate
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