Università degli Studi di Milano   Archivio Istituzionale della Ricerca
Please use this identifier to cite or link to this item: http://hdl.handle.net/2434/40119

Title: Antioxidant and radical scavenging activity of honey in endothelial cell cultures (EA.hy926)
Authors: ORIOLI, MARICA (Second author)
BERETTA, GIANGIACOMO (First author)
MAFFEI FACINO, ROBERTO (Last author)
Abstract: The therapeutic properties of honey, once considered a form of folk or preventive medicine, are acquiring importance for the treatment of acute and chronic free radical-mediated diseases (atherosclerosis, diabetes and cancer). The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. Cumene hydroperoxide (CuOOH) was used as free radical promoter. Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. These results provide unequivocal evidence that, through the synergistic action of its antioxidants, honey by reducing and removing ROS, may lower the risks and effects of acute and chronic free radical induced pathologies in vivo
Keywords: Honey ; endothelial cells ; radical scavenging activity ; antioxidant fraction ; GSH
MIUR subjects: CHIM/08 - Chimica Farmaceutica
Publication date: 2007
Digital Object Identifier (DOI): 10.1055/s-2007-981598
Citation: Antioxidant and radical scavenging activity of honey in endothelial cell cultures (EA.hy926) / G. Beretta, M. Orioli, R. Maffei Facino. - In: Planta medica. - ISSN 0032-0943. - ISSN 1439-0221. - 73:11(2007), pp. 1182-1189.
ISI identifier: 000250143800007
Scopus identifier: 35348815595
Type: Article (author)

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