Neuronal differentation is a crucial event in the regeneration of nervous tissues and adhesion on a substrate is critical for neurite extension. The initiation and guidance of a neurite rely on extra-cellular signals, such as substrate energy of adhesion. Therefore it ts of great interest to unveil the substrates characteristics that are sensed by the growth cone and translated into neurite extension. In the present work a proteomic approach is used to characterize at the molecular level PCl2 cells cultured on nanostructured Ti02 in order to evaluate how the nanoscale topography may modulate the dynamic of adhesion and control the shape and functions of cells as well as of tissues. Protein patterns and post-translational modifications are evaluated by immunochemistry and mass-spectrometry with particular emphasis on nitration, which is involved in neuronal proliferation and differentiation.
Nanostructured titanium oxide as substrate for PC12 cell differentiation / G. Tedeschi, E. Maffioli, S. Forti, A. Negri, C. Lenardi, P. Milani. ((Intervento presentato al 36. convegno FEBS Congress, Biochemistry for Tomorrow's Medicine tenutosi a Torino nel 2011.
Nanostructured titanium oxide as substrate for PC12 cell differentiation
G. TedeschiPrimo
;E. MaffioliSecondo
;S. Forti;A. Negri;C. LenardiPenultimo
;P. MilaniUltimo
2011
Abstract
Neuronal differentation is a crucial event in the regeneration of nervous tissues and adhesion on a substrate is critical for neurite extension. The initiation and guidance of a neurite rely on extra-cellular signals, such as substrate energy of adhesion. Therefore it ts of great interest to unveil the substrates characteristics that are sensed by the growth cone and translated into neurite extension. In the present work a proteomic approach is used to characterize at the molecular level PCl2 cells cultured on nanostructured Ti02 in order to evaluate how the nanoscale topography may modulate the dynamic of adhesion and control the shape and functions of cells as well as of tissues. Protein patterns and post-translational modifications are evaluated by immunochemistry and mass-spectrometry with particular emphasis on nitration, which is involved in neuronal proliferation and differentiation.Pubblicazioni consigliate
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