Post-ischemic reperfusion leads to apoptosis-linked loss of myocytes in cultured cells and in vivo. We tested the hypothesis that apoptosis develops without reperfusion in Langendorff-perfused hearts exposed to either low-flow ischemia (LFI) or hypoxia (H). Rat hearts were perfused with aminoacid-enriched Krebs- Henseleit buffer and exposed for 6 h to LFI (flow=2 ml/min, PO2=500±50mmHg, mean±SD), H (10ml/min, 120±15mmHg), or control conditions (C, 10ml/min, 500±50mmHg). At selected times, DNA-fragmentation was measured by agarose-gel electrophoresis and in situ TUNEL assay. After 6 h, the ratio (TUNEL-positi- ve)/(total nuclei) was 0.620±0.027, 0.615±0.005, 0.404±0.021 in LFI, H and C, respectively. The ratio was 0.813±0.021 in hearts exposed to 90 min global no-flow ischemia and reperfused (5 h). To assess the role of membrane-diffusible factors, separate experiments were performed recirculating the medium and exposing hearts to LFI or H as above. The degree of apoptosis was the same in both the recirculating and non-recirculating modes. Thus, apoptosis develops by similar extents and in a time-dependent fashion in crystalloid-perfused rat hearts during LFI or H at the same oxygen shortage (flow•PO2), even without the reperfusion.
Low-flow ischemia and hypoxia stimulate apoptosis in perfused rat hearts independently of reperfusion / S. Pozzi, G. Malferrari, I. Biunno, M. Samaja. - In: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 1015-8987. - 12:1(2002), pp. 39-46.
Low-flow ischemia and hypoxia stimulate apoptosis in perfused rat hearts independently of reperfusion
M. SamajaUltimo
2002
Abstract
Post-ischemic reperfusion leads to apoptosis-linked loss of myocytes in cultured cells and in vivo. We tested the hypothesis that apoptosis develops without reperfusion in Langendorff-perfused hearts exposed to either low-flow ischemia (LFI) or hypoxia (H). Rat hearts were perfused with aminoacid-enriched Krebs- Henseleit buffer and exposed for 6 h to LFI (flow=2 ml/min, PO2=500±50mmHg, mean±SD), H (10ml/min, 120±15mmHg), or control conditions (C, 10ml/min, 500±50mmHg). At selected times, DNA-fragmentation was measured by agarose-gel electrophoresis and in situ TUNEL assay. After 6 h, the ratio (TUNEL-positi- ve)/(total nuclei) was 0.620±0.027, 0.615±0.005, 0.404±0.021 in LFI, H and C, respectively. The ratio was 0.813±0.021 in hearts exposed to 90 min global no-flow ischemia and reperfused (5 h). To assess the role of membrane-diffusible factors, separate experiments were performed recirculating the medium and exposing hearts to LFI or H as above. The degree of apoptosis was the same in both the recirculating and non-recirculating modes. Thus, apoptosis develops by similar extents and in a time-dependent fashion in crystalloid-perfused rat hearts during LFI or H at the same oxygen shortage (flow•PO2), even without the reperfusion.File | Dimensione | Formato | |
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