Laboratory variation of 24 h in vitro gas production and estimated metabolizable energy values of ruminant feeds

Intra- and inter-laboratory variation of in vitro gas production and calculated metabolizable energy (ME, MJ/kg DM) values were studied using 16 test feeds in 7 laboratories. Intra-laboratory variation was low, with six of the seven laboratories having very high relationships in gas production between runs (R2 ≥ 0.96) and slopes that did not differ from unity. Inter-laboratory differences were higher with highly significant (P < 0.001) differences among laboratories in both gas production and calculated ME values. Three of the six test laboratories generated predicted ME values that did not differ from the seventh (reference) laboratory. Combining intra-laboratory variation in gas production and inter-laboratory variation in predicted ME values, three of the six test laboratories were judged acceptable overall. ME values predicted by the gas production technique by laboratories in different parts of the world cannot be considered absolute.