CIRCADIAN AND CIRCANNUAL RHYTHMS OF SEVERAL ENZYMES OF LYSOSOMAL ORIGIN IN HUMAN-PLASMA

The circadian and circannual group rhythms in the plasma concentrations of the following lysosomal enzymes were studied in women and men: β-d-N-acetylglucosaminidase, β-d-glucuronidase, β-d-glucosidase, β-d-galactosidase, α-d-galactosidase, α-l-fucosidase and α-d-mannosidase. The circadian rhythm was detected in all the tested enzymes of women, and only in α-d-galactosidase, β-d-glucosidase, α-d-mannosidase and β-d-N-acetylglucosaminidase of men. A statistically significant difference between genders in the circadian rhythm was exhibited by β-d-galactosidase, β-d-glucosidase, β-d-N-acetylglucosaminidase, β-d-glucuronidase, α-d-galactosidase and α-l-fucosidase. A circannual rhythm was detected in all the tested enzymes, with the exception of β-d-glucuronidase and β-d-N-acetylglucosaminidase, without any statistically significant difference between genders. The group rhythms of some of the enzymes (α-d-galactosidase, β-d-glucosidase, β-d-galactosidase) showed similar values of both circadian and circannual acrophases, suggesting that they may be subjected as a group to the same chronobiological coordination. The chronobiological rhythms of lysosomal enzymes were different from those of lactate dehydrogenase and α1-antitrypsin, indicating that these rhythms are not merely reflecting fluctuations of the water content of plasma. No in-phase relationship was observed between the circadian and circannual rhythms of plasma cortisol and those of the tested lysosomal enzymes, excluding a direct chronobiological relationship between this hormone and lysosomal enzymes.