AIMS: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. METHODS: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. RESULTS: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). CONCLUSION: Our findings support the use of nasal cell DNA for human epigenetic studies of asthma
Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma / A. Baccarelli, F. Rusconi, V. Bollati, D. Catelan, G. Accetta, L. Hou, F. Barbone, P.A. Bertazzi, A. Biggeri. - In: EPIGENOMICS. - ISSN 1750-1911. - 4:1(2012 Feb), pp. 91-100.
Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma
A. BaccarelliPrimo
;V. Bollati;P.A. BertazziPenultimo
;
2012
Abstract
AIMS: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. METHODS: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. RESULTS: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). CONCLUSION: Our findings support the use of nasal cell DNA for human epigenetic studies of asthmaFile | Dimensione | Formato | |
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