A two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins has been established. About 250 spots were detected after silver staining and polypeptides from 24 spots have been N-terminally sequenced. Major proteins already described in bull seminal plasma, like PDC-109 and aSFP, have been located on the map; proteins not yet reported in male reproductive tracts have been evidenced; for some polypeptides showing a previously unknown N-terminal sequence, structural similarities with proteins described in other organisms have been found. A reference map of seminal plasma proteins could be useful in relating protein pattern changes to physiopathological events influencing the reproductive sphere.
Two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins / M. Mortarino, G. Tedeschi, A. Negri, F. Ceciliani, L. Gottardi, G. Maffeo, S. Ronchi. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 5:5(1998), pp. 797-801.
Two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins
M. MortarinoPrimo
;G. TedeschiSecondo
;A. Negri;F. Ceciliani;G. MaffeoPenultimo
;S. RonchiUltimo
1998
Abstract
A two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins has been established. About 250 spots were detected after silver staining and polypeptides from 24 spots have been N-terminally sequenced. Major proteins already described in bull seminal plasma, like PDC-109 and aSFP, have been located on the map; proteins not yet reported in male reproductive tracts have been evidenced; for some polypeptides showing a previously unknown N-terminal sequence, structural similarities with proteins described in other organisms have been found. A reference map of seminal plasma proteins could be useful in relating protein pattern changes to physiopathological events influencing the reproductive sphere.Pubblicazioni consigliate
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