Phaseolin, one of the major legume proteins for human nutrition, is a trimeric glycoprotein of the 7S class that accumulates in the protein storage vacuoles of common bean. Phaseolin is cotranslationally introduced into the lumen of the endoplasmic reticulum; from there, it is transported through the Golgi complex to the storage vacuoles. Phaseolin is also transported to the vacuole in vegetative tissues of transgenic plants. By transient and permanent expression in tobacco leaf cells, we show here that vacuolar sorting of phaseolin is saturable and that saturation leads to Golgi-mediated secretion from the cell. A mutated phaseolin, in which the four C-terminal residues (Ala, Phe, Val, and Tyr) were deleted, efficiently formed trimers but was secreted entirely outside of the cells in transgenic tobacco leaves, indicating that the deleted sequence contains information necessary for interactions with the saturable vacuolar sorting machinery. In the apoplast, the secreted phaseolin remained intact; this is similar to what occurs to wild-type phaseolin in bean storage vacuoles, whereas in vegetative vacuoles of transgenic plants, the storage protein is fragmented.

Sorting of phaseolin to the vacuole is saturable and requires a short C-terminal peptide / L. Frigerio, M. de Virgilio, A. Prada, F. Faoro, A. Vitale. - In: PLANT CELL. - ISSN 1040-4651. - 10:6(1998 Jun), pp. 1031-1042.

Sorting of phaseolin to the vacuole is saturable and requires a short C-terminal peptide

F. Faoro
Penultimo
;
1998

Abstract

Phaseolin, one of the major legume proteins for human nutrition, is a trimeric glycoprotein of the 7S class that accumulates in the protein storage vacuoles of common bean. Phaseolin is cotranslationally introduced into the lumen of the endoplasmic reticulum; from there, it is transported through the Golgi complex to the storage vacuoles. Phaseolin is also transported to the vacuole in vegetative tissues of transgenic plants. By transient and permanent expression in tobacco leaf cells, we show here that vacuolar sorting of phaseolin is saturable and that saturation leads to Golgi-mediated secretion from the cell. A mutated phaseolin, in which the four C-terminal residues (Ala, Phe, Val, and Tyr) were deleted, efficiently formed trimers but was secreted entirely outside of the cells in transgenic tobacco leaves, indicating that the deleted sequence contains information necessary for interactions with the saturable vacuolar sorting machinery. In the apoplast, the secreted phaseolin remained intact; this is similar to what occurs to wild-type phaseolin in bean storage vacuoles, whereas in vegetative vacuoles of transgenic plants, the storage protein is fragmented.
Recombinant Proteins ; Plants, Genetically Modified ; Plants, Medicinal ; Humans ; Amino Acid Sequence ; Nutritional Physiological Phenomena ; Plant Proteins ; Mutagenesis, Site-Directed ; Plants, Toxic ; Sequence Alignment ; Plant Leaves ; Vacuoles ; Fabaceae ; Tobacco ; Molecular Sequence Data ; Protoplasts ; Golgi Apparatus ; Toxins, Biological
Settore BIO/11 - Biologia Molecolare
Settore BIO/10 - Biochimica
Settore AGR/12 - Patologia Vegetale
giu-1998
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/176051
Citazioni
  • ???jsp.display-item.citation.pmc??? 58
  • Scopus 148
  • ???jsp.display-item.citation.isi??? 144
social impact