The basis for intron expansion in humans is largely unexplored. In this article, we demonstrate that intron expansion has primarily been determined by fixation of multispecies conserved sequences (MCSs) over time. The presence of MCSs has shaped intron features: the insertion of transposable elements (TEs) has been constrained as more MCSs were fixed. Analysis of TE and MCS distribution suggested an unprecedented estimate of information requirements for proper splicing of long introns with indication of sequence constraints extending up to >3 kb downstream 5′ splice sites.
Fixation of conserved sequences shapes human intron size and influences transposon insertion dynamics / M. Sironi, G. Menozzi, G.P. Comi, R. Cagliani, N. Bresolin, U. Pozzoli - In: Atti del Congresso / RECOMB Satellite Workshop on Comparative Genomics. - [s.l] : RECOMB Satellite Workshop on Comparative Genomics, 2005. - pp. 484-488 (( Intervento presentato al 3. convegno RECOMB Satellite Workshop on Comparative Genomics tenutosi a Dublino nel 2005 [10.1016/j.tig.2005.06.009].
Fixation of conserved sequences shapes human intron size and influences transposon insertion dynamics
G.P. Comi;N. BresolinPenultimo
;
2005
Abstract
The basis for intron expansion in humans is largely unexplored. In this article, we demonstrate that intron expansion has primarily been determined by fixation of multispecies conserved sequences (MCSs) over time. The presence of MCSs has shaped intron features: the insertion of transposable elements (TEs) has been constrained as more MCSs were fixed. Analysis of TE and MCS distribution suggested an unprecedented estimate of information requirements for proper splicing of long introns with indication of sequence constraints extending up to >3 kb downstream 5′ splice sites.
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