Xenopus laevis Gremlin has been isolated as a novel dorsalizing factor, belonging to a family of secreted proteins with axial patterning activity [Hsu, D.R., Economides, A.N., Wang, X., Eimon, M., Harland, R.M., 1998. The Xenopus dorsalizing factor gremlin identified a novel family of secreted proteins that antagonize BMP activities. Mol. Cell 1, 673-683]. In a search for genes that control development in zebrafish (Danio rerio), we have identified a sequence homologous to Xenopus gremlin. This paper describes the cloning of zebrafish gremlin (grm) and its expression pattern during development. Our results show that grm encodes a maternal transcript, and the zygotic transcription is turned on at the mid-blastula transition (MBT), when grm is detected in the entire blastoderm. In the gastrula grm becomes restricted to the dorsolateral region of the embryo, and during somitogenesis it is strongly expressed in the presomitic mesoderm and developing somites, and in the ventral neural tube. From 24 hpf to 48 hpf, we show that grm transcription is downregulated in the whole embryo, even though Grm protein is still present and localized into the entire myotome at 48-72 hpf. Finally, grm transcript is strongly downregulated in fibroblast growth factor-8 (fgf8) and sonic hedgehog (shh) mutants, thus implicating a putative role of Fgf/Shh signalling loop in grm expression regulation. (copyright) 2004 Elsevier B.V. All rights reserved.

Regulated expression pattern of gremlin during zebrafish development / F. Cotelli, M. Presta, O. Pozzoli, C.N. Gilardelli, S. Nicoli. - In: GENE EXPRESSION PATTERNS. - ISSN 1567-133X. - 5:4(2005), pp. 539-544. [10.1016/j.modgep.2004.11.001]

Regulated expression pattern of gremlin during zebrafish development

F. Cotelli
Primo
;
2005

Abstract

Xenopus laevis Gremlin has been isolated as a novel dorsalizing factor, belonging to a family of secreted proteins with axial patterning activity [Hsu, D.R., Economides, A.N., Wang, X., Eimon, M., Harland, R.M., 1998. The Xenopus dorsalizing factor gremlin identified a novel family of secreted proteins that antagonize BMP activities. Mol. Cell 1, 673-683]. In a search for genes that control development in zebrafish (Danio rerio), we have identified a sequence homologous to Xenopus gremlin. This paper describes the cloning of zebrafish gremlin (grm) and its expression pattern during development. Our results show that grm encodes a maternal transcript, and the zygotic transcription is turned on at the mid-blastula transition (MBT), when grm is detected in the entire blastoderm. In the gastrula grm becomes restricted to the dorsolateral region of the embryo, and during somitogenesis it is strongly expressed in the presomitic mesoderm and developing somites, and in the ventral neural tube. From 24 hpf to 48 hpf, we show that grm transcription is downregulated in the whole embryo, even though Grm protein is still present and localized into the entire myotome at 48-72 hpf. Finally, grm transcript is strongly downregulated in fibroblast growth factor-8 (fgf8) and sonic hedgehog (shh) mutants, thus implicating a putative role of Fgf/Shh signalling loop in grm expression regulation. (copyright) 2004 Elsevier B.V. All rights reserved.
Can domain; Dorsoventral axis; Fibroblast growth factor; Somitogenesis; Sonic hedgehog; Zebra fish embryo
Settore BIO/06 - Anatomia Comparata e Citologia
2005
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/13313
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 11
social impact