Autism spectrum disorders (ASDs) represent a diagnostic challenge with a still partially uncertain etiology, in which genetic and environmental factors have now been assessed. Among the hypotheses underlying the involvement of biological and environmental factors, the gut–brain axis is of particular interest in autism spectrum disorders. Several studies have highlighted the related incidence of particular gastrointestinal symptoms (GISs) in children suffering from ASDs. Probiotics have shown success in treating several gastrointestinal dysbiotic disorders; therefore, it is plausible to investigate whether they can alleviate behavioral symptoms as well. On these bases, a randomized double-blind crossover study with a placebo was conducted, evaluating the effects of a mixture of probiotics in a group of 61 subjects aged between 24 months and 16 years old with a diagnosis of ASD. Behavioral evaluation was performed through the administration of a questionnaire including a Parenting Stress Index (PSI) test and the Vineland Adaptive Behavior Scale (VABS). The Psycho-Educational Profile and the Autism Spectrum Rating Scale (ASRS) were also evaluated. Microbial composition analyses of fecal samples of the two groups was also performed. The study showed significant improvements in GISs, communication skills, maladaptive behaviors, and perceived parental stress level after the administration of probiotics. Microbiome alpha diversity was comparable between treatment arms and no significant differences were found, although beta diversity results were significantly different in the treatment group between T0 and T1 time points. Streptococcus thermophilus, Bifidobacterium longum, Limosilactobacillus fermentum, and Ligilactobacillus salivarius species were identified as some of the most discriminant taxa positively associated with T1 samples. This preliminary study corroborates the relationship between intestinal microbiota and ASD recently described in the literature.

Randomized Double-Blind Crossover Study for Evaluating a Probiotic Mixture on Gastrointestinal and Behavioral Symptoms of Autistic Children / C. Guidetti, E. Salvini, M. Viri, F. Deidda, A. Amoruso, A. Visciglia, L. Drago, M. Calgaro, N. Vitulo, M. Pane, A.C. Caucino. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - 11:18(2022 Sep 06), pp. 5263.1-5263.17. [10.3390/jcm11185263]

Randomized Double-Blind Crossover Study for Evaluating a Probiotic Mixture on Gastrointestinal and Behavioral Symptoms of Autistic Children

L. Drago;M. Pane;
2022

Abstract

Autism spectrum disorders (ASDs) represent a diagnostic challenge with a still partially uncertain etiology, in which genetic and environmental factors have now been assessed. Among the hypotheses underlying the involvement of biological and environmental factors, the gut–brain axis is of particular interest in autism spectrum disorders. Several studies have highlighted the related incidence of particular gastrointestinal symptoms (GISs) in children suffering from ASDs. Probiotics have shown success in treating several gastrointestinal dysbiotic disorders; therefore, it is plausible to investigate whether they can alleviate behavioral symptoms as well. On these bases, a randomized double-blind crossover study with a placebo was conducted, evaluating the effects of a mixture of probiotics in a group of 61 subjects aged between 24 months and 16 years old with a diagnosis of ASD. Behavioral evaluation was performed through the administration of a questionnaire including a Parenting Stress Index (PSI) test and the Vineland Adaptive Behavior Scale (VABS). The Psycho-Educational Profile and the Autism Spectrum Rating Scale (ASRS) were also evaluated. Microbial composition analyses of fecal samples of the two groups was also performed. The study showed significant improvements in GISs, communication skills, maladaptive behaviors, and perceived parental stress level after the administration of probiotics. Microbiome alpha diversity was comparable between treatment arms and no significant differences were found, although beta diversity results were significantly different in the treatment group between T0 and T1 time points. Streptococcus thermophilus, Bifidobacterium longum, Limosilactobacillus fermentum, and Ligilactobacillus salivarius species were identified as some of the most discriminant taxa positively associated with T1 samples. This preliminary study corroborates the relationship between intestinal microbiota and ASD recently described in the literature.
No
English
ASD; autism spectrum disorder; autistic children; behavioral symptoms; gastrointestinal symptoms; gut–brain axis; microbiome analysis; microbiota; probiotics; randomized double-blind crossover study
Settore MED/07 - Microbiologia e Microbiologia Clinica
Articolo
Esperti anonimi
Pubblicazione scientifica
6-set-2022
MDPI
11
18
5263
1
17
17
Pubblicato
Periodico con rilevanza internazionale
scopus
orcid
pubmed
crossref
wos
Aderisco
info:eu-repo/semantics/article
Randomized Double-Blind Crossover Study for Evaluating a Probiotic Mixture on Gastrointestinal and Behavioral Symptoms of Autistic Children / C. Guidetti, E. Salvini, M. Viri, F. Deidda, A. Amoruso, A. Visciglia, L. Drago, M. Calgaro, N. Vitulo, M. Pane, A.C. Caucino. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - 11:18(2022 Sep 06), pp. 5263.1-5263.17. [10.3390/jcm11185263]
open
Prodotti della ricerca::01 - Articolo su periodico
11
262
Article (author)
Periodico con Impact Factor
C. Guidetti, E. Salvini, M. Viri, F. Deidda, A. Amoruso, A. Visciglia, L. Drago, M. Calgaro, N. Vitulo, M. Pane, A.C. Caucino
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/970615
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